Team:Paris Saclay/Protocols
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=Protocols= | =Protocols= | ||
+ | |||
+ | ==Countdown== | ||
+ | This page is under '''Maher''''s responsibility | ||
+ | |||
+ | * Deadline: 08/oct. | ||
+ | ** Uniformisation des textes. | ||
+ | ** Rappeler Fabio de creer la photo pour le protocol BioBrick Assembly | ||
+ | ** Changer les enzymes du protocol | ||
+ | * Deadline: 12/oct | ||
+ | ** Final review. | ||
+ | |||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Preparation_of_supercompetent_Ecoli_cells Preparation of supercompetent ''E. coli'' cells] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Preparation_of_supercompetent_Ecoli_cells Preparation of supercompetent ''E. coli'' cells] |
Revision as of 14:26, 4 October 2014
Protocols
Countdown
This page is under Maher's responsibility
- Deadline: 08/oct.
- Uniformisation des textes.
- Rappeler Fabio de creer la photo pour le protocol BioBrick Assembly
- Changer les enzymes du protocol
- Deadline: 12/oct
- Final review.
- Preparation of supercompetent E. coli cells
- Transformation of supercompetent E. coli cells
- Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue
- Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue
- Gel Electrophoresis
- Transformation of CaCl2 supercompetent E. coli cells
- PCR for the genomic DNA of bacteria
- PCR clean-up
- PCR for bacterial culture
- Transformation of competent cells by electroporation
- BioBrick Assembly
- P1 Phages Stock
- P1 phages transduction
- Transformation of competent E. coli by CaCl2
- Cloning in pGEMTeasy
- Electro elution of DNA from agarose gel