"
Team:Paris Saclay/Protocols
From 2014.igem.org
(Difference between revisions)
(→Protocols) |
(→Protocols) |
||
| Line 17: | Line 17: | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2 Transformation of competent ''E. coli'' by CaCl<sub>2</sub>] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2 Transformation of competent ''E. coli'' by CaCl<sub>2</sub>] | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Cloning_in_pGEMTeasy Cloning in pGEMTeasy] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Cloning_in_pGEMTeasy Cloning in pGEMTeasy] | ||
| - | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Electro | + | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Electro extraction of DNA from agarose gel] |
{{Team:Paris_Saclay/protocols_footer}} | {{Team:Paris_Saclay/protocols_footer}} | ||
Revision as of 14:39, 21 August 2014
Protocols
- Preparation of supercompetent E. coli cells
- Transformation of supercompetent E. coli cells
- Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue
- Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue
- Gel Electrophoresis
- Transformation of CaCl2 supercompetent E. coli cells
- PCR for the genomic DNA of bacteria
- PCR clean-up
- PCR for bacterial culture
- Transformation of competent cells by electroporation
- BioBrick Assembly
- P1 Phages Stock
- P1 phages transduction
- Transformation of competent E. coli by CaCl2
- Cloning in pGEMTeasy
- extraction of DNA from agarose gel
