Team:Paris Saclay/Notebook/August/20
From 2014.igem.org
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Revision as of 13:43, 20 August 2014
Wednesday 20th August
D- Lemon scent
Bacterial culture
by Laetitia
Here are the results of the bacterial culture of yesterday
Bacteria with the pGEMTeasy without the DNA insert are blue and the bacteria with the pGEMTeasy and the DNA insert (PS, GS or LS gene) are white
Then, we have chosen 3 white colonies for each DNA insert and we have launched 3 corresponding liquid cultures :
- 5mL LB + ampiciline (1/1000e)
- 1 white colony
The 9 cultures are incubated at 37°C
by Melanie
Preculture 20ml of DH5a pPSI (apra 1/2000)