"
Team:Paris Saclay/Notebook/August/19
From 2014.igem.org
(Difference between revisions)
(→Lab work) |
(→D - Lemon Scent) |
||
| Line 86: | Line 86: | ||
| + | ====Purification of ligation products==== | ||
| + | We used the pGEMTeasy containing limonen synthase prepared previously : [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18 August 18th] | ||
| + | The five samples were pooled in one and was purified using this protocol : [https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up protocol] | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 15:15, 19 August 2014
Contents |
Tuesday 19th August
Lab work
D - Lemon Scent
Digestion of PCR results
by Sean, Hoang Vu, Eugene
| components | volumes |
|---|---|
| PCR purified LS | 15 μl |
| FastDigest green buffer 10X | 2 μl |
| PacI | 1 µl |
| H2O | 2 µl |
| components | volumes |
|---|---|
| PCR purified GS | 15 μl |
| FastDigest green buffer 10X | 2 μl |
| PacI | 1 µl |
| H2O | 2 µl |
| components | volumes |
|---|---|
| PCR purified PS | 15 μl |
| FastDigest green buffer 10X | 2 μl |
| PacI | 1 µl |
| H2O | 2 µl |
| components | volumes |
|---|---|
| PCR purified pPS1 | 30 μl |
| FastDigest green buffer 10X | 4 μl |
| PacI | 2 µl |
| H2O | 4 µl |
Purification of ligation products
We used the pGEMTeasy containing limonen synthase prepared previously : August 18th The five samples were pooled in one and was purified using this protocol : protocol
