Team:Paris Saclay/Notebook/August/14

From 2014.igem.org

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=Thursday 14th August=
=Thursday 14th August=
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==Lab Work==
==Lab Work==
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue Protocol]
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==Human Pratices==
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===Art & Design===
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''by Terry''
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We found an interesting colorless medium named M63.
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Preparation of M63 medium, concentration 5X, one liter basis :
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*10g (NH4)2 SO4
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*68g KH2 PO4
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*2.5mg FeSO4 7H2O
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*Adjust to pH 7 with KOH
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The concentrated medium is diluted to 1X with sterile water and the following solution ( one liter basis ) :
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*1 ml 1M MgSO4 7H2O
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*10 ml 20% carbon source ( sugar or glycerol )
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*0.1 ml 0.5% vitamin B1
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100 ml of M63 has been prepared for futur manipulations.
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 +
 +
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 15:04, 18 August 2014

Contents

Thursday 14th August

Lab Work

by Melanie

Preparation of DH5a CaCl2 Competent cell

Protocol

D-Lemon Scent

by Melanie

From the 20 ml of odor free culture (pJBEI Apra) done yesterday (by Laetitia)

Plasmide extraction without Nucleospin


Transformation of DH5 alpha

by Sean & Eugene We prepare a LB agar environment with a concentration of:

  • 0.5 mM of IPTG
  • 0.008% of Xgal
  • 1/2000 of Ampicilline

and we spread our preparation in a petri dish and incubate overnight at 37°C.


Protocol


Human Pratices

Art & Design

by Terry

We found an interesting colorless medium named M63.

Preparation of M63 medium, concentration 5X, one liter basis :

  • 10g (NH4)2 SO4
  • 68g KH2 PO4
  • 2.5mg FeSO4 7H2O
  • Adjust to pH 7 with KOH

The concentrated medium is diluted to 1X with sterile water and the following solution ( one liter basis ) :

  • 1 ml 1M MgSO4 7H2O
  • 10 ml 20% carbon source ( sugar or glycerol )
  • 0.1 ml 0.5% vitamin B1

100 ml of M63 has been prepared for futur manipulations.


Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/August/14"