Team:Paris Saclay/Notebook/August/13

From 2014.igem.org

(Difference between revisions)
(→Cloning BBa_K517003 and p cola in pGEMTeasy)
(→Cloning BBa_K517003 and p cola in pGEMTeasy)
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|DNA insert
|DNA insert
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|4.7 µl of 16.8 ng/µl
+
|4.7 µl at 16.8 ng/µl
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|2T4DNA ligase
|2T4DNA ligase
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|-
|-
|DNA insert
|DNA insert
-
|5.6 µl of 16.8 ng/µl
+
|5.6 µl at 16.8 ng/µl
|-
|-
|2T4DNA ligase
|2T4DNA ligase

Revision as of 13:37, 14 August 2014

Contents

Wednesday 13th August

Lab work

D - Lemon scent

Gel electrophoresis of BBa_K762100

by Sean

Cloning BBa_K517003 and p cola in pGEMTeasy

by Eugene and Sean

P cola and BBa_K517003 cloning
components volumes
PCR purified p cola/ BBa_K517003 7 μl
DreamTaq buffer 10X 1 μl
dATP 0.2 mM
DreamTaq 5 units

incubate for 30 minutes at 70°C

Geraniol synthase
components volumes
2xT4ligase buffer 10 μl
pGEMTeasy 1 μl
DNA insert 4.7 µl at 16.8 ng/µl
2T4DNA ligase 1 µl
H2O 3.3 µl
Pinene synthase
components volumes
2xT4ligase buffer 10 μl
pGEMTeasy 1 μl
DNA insert 5.6 µl at 16.8 ng/µl
2T4DNA ligase 1 µl
H2O 2.4 µl


Back to the calendar

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