Team:Paris Saclay/Notebook/August/13

From 2014.igem.org

(Difference between revisions)
(→Cloning BBa_K517003 and p cola in pGEMTeasy)
(→Cloning BBa_K517003 and p cola in pGEMTeasy)
Line 9: Line 9:
====Cloning BBa_K517003 and p cola in pGEMTeasy====
====Cloning BBa_K517003 and p cola in pGEMTeasy====
''by Eugene and Sean''
''by Eugene and Sean''
-
 
{| class="wikitable centre" width="50%"
{| class="wikitable centre" width="50%"
-
|+ P cola cloning
+
|+ P cola and BBa_K517003 cloning  
|-
|-
! scope=col | components
! scope=col | components
! scope=col | volumes
! scope=col | volumes
|-
|-
-
|PCR purified p cola  
+
|PCR purified p cola/ BBa_K517003
-
|? μl
+
|7 μl
|-
|-
|DreamTaq buffer 10X
|DreamTaq buffer 10X
Line 30: Line 29:
|}
|}
 +
incubate for 30 minutes at 70°C
{| class="wikitable centre" width="50%"
{| class="wikitable centre" width="50%"
-
|+ BBa_K517003 cloning
+
|+ Geraniol synthase
|-
|-
! scope=col | components
! scope=col | components
! scope=col | volumes
! scope=col | volumes
|-
|-
-
|PCR purified BBa_K517003
+
|2xT4ligase buffer
-
|? μl
+
|10 μl
|-
|-
-
|DreamTaq buffer 10X
+
|pGEMTeasy
|1 μl
|1 μl
|-
|-
-
|dATP
+
|DNA insert
-
|0.2 mM
+
|4.7 µl of 16.8 ng/µl
|-
|-
-
|DreamTaq
+
|2T4DNA ligase
-
|5 units
+
|1 µl
 +
|-
 +
|H<sub>2</sub>O
 +
|3.3 µl
|}
|}
 +
 +
{| class="wikitable centre" width="50%"
 +
|+ Pinene synthase
 +
|-
 +
! scope=col | components
 +
! scope=col | volumes
 +
|-
 +
|2xT4ligase buffer
 +
|10 μl
 +
|-
 +
|pGEMTeasy
 +
|1 μl
 +
|-
 +
|DNA insert
 +
|5.6 µl of 16.8 ng/µl
 +
|-
 +
|2T4DNA ligase
 +
|1 µl
 +
|-
 +
|H<sub>2</sub>O
 +
|2.4 µl
 +
|}
 +
{{Team:Paris_Saclay/notebook_footer}}
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 13:35, 14 August 2014

Contents

Wednesday 13th August

Lab work

D - Lemon scent

Gel electrophoresis of BBa_K762100

by Sean

Cloning BBa_K517003 and p cola in pGEMTeasy

by Eugene and Sean

P cola and BBa_K517003 cloning
components volumes
PCR purified p cola/ BBa_K517003 7 μl
DreamTaq buffer 10X 1 μl
dATP 0.2 mM
DreamTaq 5 units

incubate for 30 minutes at 70°C

Geraniol synthase
components volumes
2xT4ligase buffer 10 μl
pGEMTeasy 1 μl
DNA insert 4.7 µl of 16.8 ng/µl
2T4DNA ligase 1 µl
H2O 3.3 µl
Pinene synthase
components volumes
2xT4ligase buffer 10 μl
pGEMTeasy 1 μl
DNA insert 5.6 µl of 16.8 ng/µl
2T4DNA ligase 1 µl
H2O 2.4 µl


Back to the calendar

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