Team:Paris Saclay/Notebook/July/8

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(Tuesday 8th July)
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
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'''Members present:'''
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* Instructors and advisors: Alice, Solenne and Sylvie.
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* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
{{Team:Paris_Saclay/notebook_footer}}
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 14:38, 7 August 2014

Contents

Tuesday 8th July

Lab work

Rehydration of the following biobricks:

Sean

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)

Protocol:

  1. Pierce foil cover of well containing biobrick of interest.
  2. Add 10 μl of water in well.
  3. Transfer contents of well into a 1.5 ml microcentrifuge tube.

Transformation in DH5α of the following biobricks:

Mathieu & Maher

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)
  • Control + : pUC18 (20ng)
  • Control -

Protocol

NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.

Plasmid DNA preparation:

Romain & Sean

Plasmids used:

  • pJBEI-6409

E. coli strain used:

  • XL1-Blue

Protocol

Members present:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

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