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Team:Paris Saclay/Notebook
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==Planning== | ==Planning== | ||
| + | ===A - The chassis coli Odor free=== | ||
| + | *Culture of the supercompetent cells MG1655 and MG1655Z1 [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June] | ||
| + | *Phages preparation for the transduction [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July] | ||
| + | *Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 [3rd July] | ||
| + | *Cultures of MG1655 and MG1655Z1 [4th July] | ||
| + | *Transformations test of competent cells MG1655 and MG1655Z1 [10th July] | ||
| + | *Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria [11th July] | ||
| + | *Preparation of competent cells E. coli MG1655 and MG1655Z1 [17th July] | ||
| + | *Transformation of supercompetent cells MG1655 and MG1655Z1 [18th July] | ||
| + | *Results of the transformation [21st July] | ||
| + | *Preparation and transformation of competent MG1655 and MG1655Z1 [22nd July] | ||
| + | *PCR verification of the strains grown [25th July] | ||
| + | *Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 [28th July] | ||
| + | *PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R [29th July] | ||
| + | *Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 [30th July] | ||
| + | *Stria of MG1655 and MG1655Z1 on LB dishes [1st August] | ||
| + | *Stria of MG1655 and MG1655Z1 on LB and kan dishes [4th August] | ||
| + | *PCR verification of the final strains MG1655 and MG1655Z1 [5th August] | ||
| + | *Final stock of MG1655 [6th August] | ||
===C - Salicylate Inducible Suppressing System=== | ===C - Salicylate Inducible Suppressing System=== | ||
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July] | *Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July] | ||
Revision as of 08:49, 7 August 2014
Contents |
Notebook
We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page
Planning
A - The chassis coli Odor free
- Culture of the supercompetent cells MG1655 and MG1655Z1 30th June
- Phages preparation for the transduction 2nd July
- Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 [3rd July]
- Cultures of MG1655 and MG1655Z1 [4th July]
- Transformations test of competent cells MG1655 and MG1655Z1 [10th July]
- Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria [11th July]
- Preparation of competent cells E. coli MG1655 and MG1655Z1 [17th July]
- Transformation of supercompetent cells MG1655 and MG1655Z1 [18th July]
- Results of the transformation [21st July]
- Preparation and transformation of competent MG1655 and MG1655Z1 [22nd July]
- PCR verification of the strains grown [25th July]
- Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 [28th July]
- PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R [29th July]
- Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 [30th July]
- Stria of MG1655 and MG1655Z1 on LB dishes [1st August]
- Stria of MG1655 and MG1655Z1 on LB and kan dishes [4th August]
- PCR verification of the final strains MG1655 and MG1655Z1 [5th August]
- Final stock of MG1655 [6th August]
C - Salicylate Inducible Suppressing System
- Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23th July
- Transformation of competent E.coli cells - 24th July
- Bacterial culture - 25th July
- Plasmid DNA Purification - 28th July
- Digestion to check & Electrophoresis - 28th July
- Main Digestion - 29th July
- Segregate Process - 29th July
- DNA extraction from gel agarose - 30th July
- Ligation - 30th July
- Transformation of competent E.coli cells - 31st July
- Liquid Culture - 1st August
- Plasmid DNA Purification - 4th August
- Digestion to check & Electrophoresis - 4th August
- Final Stock BBa_K1372000 - 4th August
- Liquid Culture - 5th August
- Plasmid DNA Purification - 6th August
- Digestion of BBa_B0015 and BBa_K1372000 - 6th August
- Segregate Process - 6th August
- DNA extraction from gel agarose - 7th August
- Ligation - 7th August
- Transformation of competent E.coli cells - 8th August
- Liquid Culture - 11th August
- Plasmid DNA Purification - 12th August
- Digestion to check & Electrophoresis - 12th August
- Final Stock - 12th August
D - Lemon Scent
- PCR of BBa_517003 and BBa_K762100 - 4th August
- Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
- PCR Cleanup of BBa_K517003 and BBa_K762100 - 6th August
