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Team:Paris Saclay/Notebook/July/31
From 2014.igem.org
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=Thursday 31st July= | =Thursday 31st July= | ||
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==Lab work== | ==Lab work== | ||
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===C - Salicylate Inducible Suppressing System=== | ===C - Salicylate Inducible Suppressing System=== | ||
====Transformation of competent E.coli cells==== | ====Transformation of competent E.coli cells==== | ||
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'''Samples:''' | '''Samples:''' | ||
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# Sample 2 μl | # Sample 2 μl | ||
# Sample 1 μl | # Sample 1 μl | ||
# DH5α Control | # DH5α Control | ||
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'''Results on 1st August morning:''' | '''Results on 1st August morning:''' | ||
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# Success, grown very well (+- 100 colonies) | # Success, grown very well (+- 100 colonies) | ||
# Success, grown well (+- 50 colonies) | # Success, grown well (+- 50 colonies) | ||
# Success, nothing has grown | # Success, nothing has grown | ||
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
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* Students: Arnaud, Fabio, Romain, Sean and Terry. | * Students: Arnaud, Fabio, Romain, Sean and Terry. | ||
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Revision as of 08:30, 7 August 2014
Contents |
Thursday 31st July
Lab work
C - Salicylate Inducible Suppressing System
Transformation of competent E.coli cells
by Fabio
We transformed in DH5α the Ligation's result made yesterday (BBa_J61051 with BBa_K228001).
Samples:
- Sample 2 μl
- Sample 1 μl
- DH5α Control
Results on 1st August morning:
- Success, grown very well (+- 100 colonies)
- Success, grown well (+- 50 colonies)
- Success, nothing has grown
D - Lemon scent
by Romain
2 Precultures of DY330 with pJBEI-6409 in 2ml LB and Cm.
Human Pratices
Reunion, Handled topics:
- Organisation of the Meeting with french teams
- Great discussion about Human Practices
- Discussion about the logo
Members present:
- Instructors and advisors: Alice and Solenne.
- Students: Arnaud, Fabio, Romain, Sean and Terry.
