Team:Paris Saclay/Notebook/July/23

From 2014.igem.org

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{{Team:Paris_Saclay/notebook_header}}
=Wednesday 23rd July=
=Wednesday 23rd July=
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==Lab work==
==Lab work==
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===C - The Suppressing Salicylate System===
===C - The Suppressing Salicylate System===
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====Rehydration of BioBricks====
====Rehydration of BioBricks====
''by Fabio''
''by Fabio''
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===D - Lemon scent===
===D - Lemon scent===
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====p cola part 2====
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====1 - p cola part 2====
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''by Sean''
''by Sean''
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# <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font>
# <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font>
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==Reunion==
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==Modeling==
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Meeting of modeling with advisor Claire to talk about:
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* Diffusion of O<sub>2</sub> in the gel
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* Bacterial growth on the surface
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* translecture system
 +
* Diffusion of the limonene
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==Reunion==
Handled topics:
Handled topics:
* Collaborations with Colombia, Paris Bettencourt and Virginia
* Collaborations with Colombia, Paris Bettencourt and Virginia
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* Instructors and advisors: Solenne and Sylvie.
* Instructors and advisors: Solenne and Sylvie.
* Students: Arnaud, Eugene, Fabio, Juliette, Leila, Pierre, Romain, Sean and Terry.
* Students: Arnaud, Eugene, Fabio, Juliette, Leila, Pierre, Romain, Sean and Terry.
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{{Team:Paris_Saclay/notebook_footer}}
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+
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meeting of modeling with advisor Claire to talk about
+
-
* Diffusion of O<sub>2</sub> in the gel
+
-
* Bacterial growth on the surface
+
-
* translecture system
+
-
* Diffusion of the limonene
+
-
 
+
-
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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Revision as of 08:42, 7 August 2014

Contents

Wednesday 23rd July

Lab work

C - The Suppressing Salicylate System

Rehydration of BioBricks

by Fabio

The first step of this Subproject is to rehydrate the following BioBricks. Both provided in the iGEM 2014 DNA Kit Distribution.

  • BBa_J61051 - Salicylate promoter + NahR
  • BBa_K228001 - RNA suppressor

D - Lemon scent

p cola part 2

by Sean

p cola DNA was extracted in several instances yesterday. Today we attempt to collect all of the DNA and precipitate it.

Protocol

  1. Add equal parts plasmid DNA and ethanol 100% in a 1.5 microcentrifuge tube.
  2. If total volume is V, then add V/5 of sodium acetate (initial concentration of NaAc is 3M; we want a final concentration of .3M). Numerical application: Total volume of p cola was 150μl. Hence 150 μl of ethanol 100% was added, as well as 30μl of NaAc 3M. Incubate at -20°C for 30 minutes. (5:20pm - 5:50pm in our case)
  3. Centrifuge for 10 minutes at 4°C and at 11000g.
  4. Discard supernatant.
  5. Add 1ml of ethanol 70%.
  6. Repeat step 3.
  7. Discard supernatant. Make tube interior as dry as possible. Tip: use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. Note: we decided to leave the tube at room temperature and continue tomorrow instead.
  8. (tomorrow) Resuspend pellet in 30μl H2O milliQ.

Modeling

Meeting of modeling with advisor Claire to talk about:

  • Diffusion of O2 in the gel
  • Bacterial growth on the surface
  • translecture system
  • Diffusion of the limonene

Reunion

Handled topics:

  • Collaborations with Colombia, Paris Bettencourt and Virginia
  • Organisation of the Meeting with french teams
  • Definitions of the sub projects
  • Definitions of notebook's standards
  • Organisation of the Wiki
  • Great discussion about Human Practices

Members there:

  • Instructors and advisors: Solenne and Sylvie.
  • Students: Arnaud, Eugene, Fabio, Juliette, Leila, Pierre, Romain, Sean and Terry.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/23"