Team:Exeter/LabBook

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<h1>Lab Book</h1>
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<p>These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.</p>
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Revision as of 01:53, 18 October 2014

Exeter | ERASE

Lab Book

On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.


Page No. Date: Description: Carried out by:
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%281%29_.JPG”>1</a> 30/06/14 Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed. Martyn Bennet, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%282%29_.JPG”>2</a> 31/06/14 Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed. Beth Hickton, Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%283%29_.JPG”>3</a> 01/07/14 List of promoters transformed, cont. Transformations were plated. Beth Hickton, Martyn Bennet
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%284%29_.JPG”>4</a> 02/07/14 The previous day transformations were plated. Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%285%29_.JPG”>5</a> 02/07/14 Transformation of iGEM re-suspended promoters. Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%286%29_.JPG”>6</a> 02/07/14 Preparation of competent E. coli cells Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%287%29_.JPG”>7</a> 03/07/14 Promoter transformation results. Jessica Rollit, Martyn Bennet
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%288%29_.JPG”>8</a> 03/07/14 E. coli growth rate. Edward Muir, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%289%29_.JPG”>9</a> 04/07/14 Transformation of promoters and gene constructs. Edward Muir, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2810%29_.JPG”>10</a> 07/07/14 Putting constructs into the iGEM vector and testing for transformation efficiency. Martyn Bennet, Edward Muir, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2811%29_.JPG”>11</a> 08/07/14 Repeat construct addition into iGEM vector and testing transformation efficiency. Edward Muir, Beth Hickton, Martyn Bennet
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2812%29_.JPG”>12</a> 08/07/14 Transforming constructs into Top10 and DH5. Beth Hickton, Martyn Bennet, Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2813%29_.JPG”>13</a> 09/07/14 Results of Construction of iGEM vector. Jessica Rollit, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2814%29_.JPG”>14</a> 10/07/14 A range of mini-preps Jessica Rollit, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2815%29_.JPG”>15</a> 11/07/14 Measuring the length of DNA. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2816%29_.JPG”>16</a> 11/07/14 Measuring the length of DNA, cont. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2817%29_.JPG”>17</a> 11/07/14 Mini-prepping picked cultures containing promoters. Callum Bailey, Elize Hernandez
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2818%29_.JPG”>18</a> 11/07/14 Parts sent for sequencing Callum Bailey, Elize Hernandez
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2819%29_.JPG”>19</a> 11/07/14 Linearizing DNA. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2820%29_.JPG”>20</a> 11/07/14 Creating glycerol stocks. Beth Hickton, Max Smart
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2821%29_.JPG”>21</a> 14/07/14 GFP and RFP resuspension and transformation. Edward Muir, Martyn Bennet, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2822%29_.JPG”>22</a> 14/07/14 GFP and RFP resuspension and transformation, cont. Beth Hickton, Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2823%29_.JPG”>23</a> 16/07/14 Mini-prepping GFP and RFP. Jessica Rollit, Edward Muir, Fran Penrose
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2824%29_.JPG”>24</a> 16/07/14 GFP and RFP digestion/ligation to PSB1C3. Beth Hickton, Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2825%29_.JPG”>25</a> 16/07/14 Plasmid digestions. Edward Muir, Callum Bailey, Benjamin Miller
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2826%29_.JPG”>26</a> 16/07/14 RFP/GFP ligations. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2827%29_.JPG”>27</a> 16/07/14 RFP/GFP ligations, cont. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2828%29_.JPG”>28</a> 17/07/14 Transformation of GFP/RFP with terminator. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2829%29_.JPG”>29</a> 18/07/14 Making LB agar for promoters experiment. Katie Pearce
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2830%29_.JPG”>30</a> 18/07/14 Promoter experiment – quantities. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2831%29_.JPG”>31</a> 18/07/14 Promoter experiment – quantities, cont. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2832%29_.JPG”>32</a> 18/07/14 Promoter experiment – quantities, cont. 2. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2833%29_.JPG”>33</a> 21/07/14 Promoter experiment preliminary timetable. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2834%29_.JPG”>34</a> 22/07/14 Electrophoresis of RFP and GFP (with terminators). Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2835%29_.JPG”>35</a> 22/07/14 Preparation of competent E. coli cells. Beth Hickton, Peter Reader
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2836%29_.JPG”>36</a> 23/07/14 GFP/RFP + terminator liquid broths mini-prepped. Beth Hickton, Peter Reader, Jessica Rollit
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2837%29_.JPG”>37</a> 23/07/14 Potassium Chromate preparation. Peter Reader, Benjamin Miller, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2838%29_.JPG”>38</a> 23/07/14 Transformation of other promoters. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2839%29_.JPG”>39</a> 24/07/14 Moving GFP+T and RFP+T onto AMP backbones. Edward Muir, Katie Pearce
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2840%29_.JPG”>40</a> 24/07/14 Ligation of GFP+T and RFP+T onto AMP backbones. Edward Muir, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2841%29_.JPG”>41</a> 24/07/14 Potassium Dichromate colorimetry and spectrophotometry. Beth Hickton, Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2842%29_.JPG”>42</a> 28/07/14 Plating Anderson RBSs. Beth Hickton, Edward Muir, Elize Hernandez
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2843%29_.JPG”>43</a> 28/07/14 Mini-prep of reporters, promoters and RBSs. Beth Hickton, Elize Hernandez, Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2844%29_.JPG”>44</a> 29/07/14 COSHH Form created for Nitroglycerine experiments. Beth Hickton, Peter Reader, Benjamin Miller
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2845%29_.JPG”>45</a> 30/07/14 Cr(VI) / Ligand experimentation. Peter Reader
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2846%29_.JPG”>46</a> 31/07/14 Mini-prep of constructs. Edward Muir, Elize Hernandez, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2847%29_.JPG”>47</a> 31/07/14 Digestion and ligation of promoter/RBS combos. Edward Muir, Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2848%29_.JPG”>48</a> 31/07/14 Oligo preparation. Edward Muir, Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2849%29_.JPG”>49</a> 31/07/14 Ligation of promoter and RBs digestions. Edward Muir, Callum Bailey, Benjamin Miller
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2850%29_.JPG”>50</a> 01/08/14 Digestion and Ligation of constructs 004 and 007. Edward Muir, Jessica Rollit, Beth Hickton
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2851%29_.JPG”>51</a> 01/08/14 Digestion and Ligation of constructs 004 and 007, cont. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2852%29_.JPG”>52</a> 05/08/14 Colonies picked and mini-prepped. Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2853%29_.JPG”>53</a> 05/08/14 Colonies picked and mini-prepped, cont. Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2854%29_.JPG”>54</a> 06/08/14 Testing promoter/RBS combination efficiency. Callum Bailey, Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2855%29_.JPG”>55</a> 06/08/14 Testing promoter/RBS combination efficiency, cont. Callum Bailey, Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2856%29_.JPG”>56</a> 07/08/14 Picking colonies from promoter/RBS combination transformations. Edward Muir, Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2857%29_.JPG”>57</a> 07/08/14 T. Cam experiment. Edward Muir, Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2858%29_.JPG”>58</a> 11/08/14 Promoter/RBS combinations again. Edward Muir, Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2859%29_.JPG”>59</a> 11/08/14 Promoter/RBS combinations again, cont. Edward Muir, Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2860%29_.JPG”>60</a> 11/08/14 Inoculation of enzymes for construct 001 and 003. Edward Muir, Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2861%29_.JPG”>61</a> 12/08/14 Inoculation of enzymes for construct 001 and 003, cont. Edward Muir, Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2862%29_.JPG”>62</a> 12/08/14 Transformation of a sample promoter/RBS combination. Callum Bailey
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2863%29_.JPG”>63</a> 12/08/14 T. Cam experiment for solvents and ether. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2864%29_.JPG”>64</a> 13/08/14 Combination transformation results and construct testing. Edward Muir
<a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2865%29_.JPG”>65</a> 13/08/14 Putting oligos into GFP and RFP. Edward Muir

These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.

Exeter | ERASE

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