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Team:Paris Saclay/Project
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== Scientific part== | == Scientific part== | ||
| - | Our project is based on the construction of a | + | Our project is based on the construction of a bacterial lemon that will look like lemon but is made only of ''Escherichia coli'' modified to produce a lemon scent and to change colour from green to yellow. We will achieve our project by genetically modifying ''Escherichia coli'' to be devoid of any unpleasant odour by deleting the genes involved in ''E. coli'''s odor, by introducing genes for the production of the lemon scent and finally by adding a yellow-blue chromoprotein whose expression is controlled by a tRNA suppressor for the colour switching. |
===[https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli Remove the bad smell of ''E.coli'']=== | ===[https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli Remove the bad smell of ''E.coli'']=== | ||
| - | Our lemon should smell like a lemon. Using ''Escherichia coli'' as a chassis, | + | Our lemon should smell like a lemon. Using ''Escherichia coli'' as a chassis, a bacterium known to have a foul odor, we have to remove the genes involved in this particular phenotype. tnaA, which is one of that gene, is required for the degradation of tryptophane into indole the main element that provide that smell. We use a phage transduction method to reach our goal. |
===[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent Lemon Scent]=== | ===[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent Lemon Scent]=== | ||
Revision as of 20:55, 15 October 2014
Contents |
Project
RAJOUTER PRESENTATION PROJET [Rémi] : de la question éthique à la réalisation du citron, en passant par l'œuvre d'art.
In a collaboration with iGEM Paris-Bettencourt team in our iGEM French Meeting, we made a video to introduce the project.
Countdown
This page is under Marie's responsibility
- Deadline: 08/oct.
- General introduction of the project.
- Introduction text of each part of the project
- Sean's video (bettencourt)
- Deadline: 12/oct
- Final review par Sylvie.
Inspirations
Scientific part
Our project is based on the construction of a bacterial lemon that will look like lemon but is made only of Escherichia coli modified to produce a lemon scent and to change colour from green to yellow. We will achieve our project by genetically modifying Escherichia coli to be devoid of any unpleasant odour by deleting the genes involved in E. coli's odor, by introducing genes for the production of the lemon scent and finally by adding a yellow-blue chromoprotein whose expression is controlled by a tRNA suppressor for the colour switching.
Remove the bad smell of E.coli
Our lemon should smell like a lemon. Using Escherichia coli as a chassis, a bacterium known to have a foul odor, we have to remove the genes involved in this particular phenotype. tnaA, which is one of that gene, is required for the degradation of tryptophane into indole the main element that provide that smell. We use a phage transduction method to reach our goal.
Lemon Scent
The particular odor of lemon comes from 3 main monoterpenes: limonene, beta-pinene and geraniol. Cloning these 3 monoterpene-synthase into our bacteria, we allow it to produce this fragrance. It requires also to improve the production of the precursor GPP using the mevalonate-pathway from Eukaryota organism.
Lemon appearance and Ripening
Since vision is one of the most important senses in humans, we want the lemon look like as real as possible. To achieve this, we planned to make our lemon look green or yellow, as a real one. Furthermore, we also wanted to simulate the ripening process of the lemon by changing its color gradually from green to yellow.
Artistic Part
Lemon Shaping
Boston Installation
Countdown
This page is under Leïla's responsibility
- Deadline: 08/oct.
- Text
- Deadline: 12/oct
- Final review by Solenne.
