Thursday 14th August

Lab Work

by Melanie

Preparation of DH5a CaCl2 Competent cell

Protocol

Lemon Scent

by Melanie

From the 20 ml of odor free culture (pJBEI Apra - pPSI) done yesterday (by Laetitia)

Plasmid extraction without Nucleospin protocol

+ Stock (DH5a pJBEI + apra)

Transformation of DH5α

by Sean & Eugene

(Cacl2 protocol)

We prepare a LB agar environment with a concentration of:

• 0.5 mM of IPTG
• 0.008% of Xgal
• 1/2000 of Ampicilline

and we spread our preparation in a petri dish and incubate overnight at 37°C.

Human Practices

Art & Design

by Terry

We found an interesting colorless medium named M63.

Preparation of M63 medium, concentration 5X, one liter basis :

• 10g (NH₄)₂ SO₄
• 68g KH₂ PO₄
• 2.5mg FeSO₄ 7H₂O
• Adjust to pH 7 with KOH

The concentrated medium is diluted to 1X with sterile water and the following solution ( one liter basis ) :

• 1 ml 1M MgSO₄ 7H₂O
• 10 ml 20% carbon source (sugar or glycerol)
• 0.1 ml 0.5% vitamin B1

100 ml of M63 has been prepared for future manipulations.

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