Team:Exeter/LabBook

From 2014.igem.org

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<h1>Lab Book</h1>
<h1>Lab Book</h1>
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<p>On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.</P>
+
<p>On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.</p>
<br>
<br>
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<table align="right">
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<table>
 +
<tr>
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   <th style="width:100px">Page No.</th>
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   <td style="width:100px;font-weight:bold;">Page No.</td>
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   <th style="width:200px">Date:</th>
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   <td style="width:200px;font-weight:bold;">Date:</td>
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   <th style="width:400px">Description:</th>  
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   <td style="width:400px;font-weight:bold;">Description:</td>  
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   <th style="width:300px">Carried out by:</th>
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   <td style="width:300px;font-weight:bold;">Carried out by:</td>
 +
</tr>
<tr>
<tr>
 +
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%281%29_.JPG | 1]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%281%29_.JPG | 1]]</td>
   <td>30/06/14</td> <!-- date -->
   <td>30/06/14</td> <!-- date -->
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<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%282%29_.JPG | 2]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%282%29_.JPG | 2]]</td>
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   <td></td>  
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   <td>31/06/14</td>  
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   <td></td>
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   <td>Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed.</td>
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   <td></td>
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   <td>Beth Hickton, Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%283%29_.JPG | 3]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%283%29_.JPG | 3]]</td>
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   <td></td>  
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   <td>01/07/14</td>  
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   <td></td>
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   <td>List of promoters transformed, cont. Transformations were plated.</td>
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   <td></td>
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   <td>Beth Hickton, Martyn Bennet</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%284%29_.JPG | 4]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%284%29_.JPG | 4]]</td>
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   <td></td>  
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   <td>02/07/14</td>  
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   <td></td>
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   <td>The previous day transformations were plated. </td>
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   <td></td>
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   <td>Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%285%29_.JPG | 5]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%285%29_.JPG | 5]]</td>
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   <td></td>  
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   <td>02/07/14</td>  
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   <td></td>
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   <td>Transformation of iGEM re-suspended promoters.</td>
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   <td></td>
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   <td>Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%286%29_.JPG | 6]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%286%29_.JPG | 6]]</td>
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   <td></td>  
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   <td>02/07/14</td>  
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   <td></td>
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   <td>Preparation of competent E. coli cells</td>
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   <td></td>
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   <td>Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%287%29_.JPG | 7]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%287%29_.JPG | 7]]</td>
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   <td></td>  
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   <td>03/07/14</td>  
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   <td></td>
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   <td>Promoter transformation results.</td>
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   <td></td>
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   <td>Jessica Rollit, Martyn Bennet</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%288%29_.JPG | 8]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%288%29_.JPG | 8]]</td>
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   <td></td>  
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   <td>03/07/14</td>  
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   <td></td>
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   <td>E. coli growth rate.</td>
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   <td></td>
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   <td>Edward Muir, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%289%29_.JPG | 9]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%289%29_.JPG | 9]]</td>
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   <td></td>  
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   <td>04/07/14</td>  
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   <td></td>
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   <td>Transformation of promoters and gene constructs.</td>
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   <td></td>
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   <td>Edward Muir, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2810%29_.JPG | 10]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2810%29_.JPG | 10]]</td>
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   <td></td>  
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   <td>07/07/14</td>  
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   <td></td>
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   <td>Putting constructs into the iGEM vector and testing for transformation efficiency.</td>
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   <td></td>
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   <td>Martyn Bennet, Edward Muir, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2811%29_.JPG | 11]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2811%29_.JPG | 11]]</td>
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   <td></td>  
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   <td>08/07/14</td>  
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   <td></td>
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   <td>Repeat construct addition into iGEM vector and testing transformation efficiency.</td>
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   <td></td>
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   <td>Edward Muir, Beth Hickton, Martyn Bennet</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2812%29_.JPG | 12]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2812%29_.JPG | 12]]</td>
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   <td></td>  
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   <td>08/07/14</td>  
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   <td></td>
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   <td>Transforming constructs into Top10 and DH5.</td>
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   <td></td>
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   <td>Beth Hickton, Martyn Bennet, Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2813%29_.JPG | 13]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2813%29_.JPG | 13]]</td>
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   <td></td>  
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   <td>09/07/14</td>  
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   <td></td>
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   <td>Results of Construction of iGEM vector.</td>
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   <td></td>
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   <td>Jessica Rollit, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2814%29_.JPG | 14]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2814%29_.JPG | 14]]</td>
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   <td></td>  
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   <td>10/07/14</td>  
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   <td></td>
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   <td>A range of mini-preps</td>
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   <td></td>
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   <td>Jessica Rollit, Beth Hickton</td>
</tr>
</tr>
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<tr
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<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2815%29_.JPG | 15]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2815%29_.JPG | 15]]</td>
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   <td></td>  
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   <td>11/07/14</td>  
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   <td></td>
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   <td>Measuring the length of DNA.</td>
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   <td></td>
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   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2816%29_.JPG | 16]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2816%29_.JPG | 16]]</td>
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   <td></td>  
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   <td>11/07/14</td>  
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   <td></td>
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   <td>Measuring the length of DNA, cont.</td>
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   <td></td>
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   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2817%29_.JPG | 17]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2817%29_.JPG | 17]]</td>
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   <td></td>  
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   <td>11/07/14</td>  
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   <td></td>
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   <td>Mini-prepping picked cultures containing promoters.</td>
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   <td></td>
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   <td>Callum Bailey, Elize Hernandez</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2818%29_.JPG | 18]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2818%29_.JPG | 18]]</td>
-
   <td></td>  
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   <td>11/07/14</td>  
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   <td></td>
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   <td>Parts sent for sequencing</td>
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   <td></td>
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   <td>Callum Bailey, Elize Hernandez</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2819%29_.JPG | 19]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2819%29_.JPG | 19]]</td>
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   <td></td>  
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   <td>11/07/14</td>  
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   <td></td>
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   <td>Linearizing DNA.</td>
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   <td></td>
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   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2820%29_.JPG | 20]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2820%29_.JPG | 20]]</td>
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   <td></td>  
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   <td>11/07/14</td>  
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   <td></td>
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   <td>Creating glycerol stocks.</td>
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   <td></td>
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   <td>Beth Hickton, Max Smart</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2821%29_.JPG | 21]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2821%29_.JPG | 21]]</td>
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   <td></td>  
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   <td>14/07/14</td>  
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   <td></td>
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   <td>GFP and RFP resuspension and transformation.</td>
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   <td></td>
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   <td>Edward Muir, Martyn Bennet, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2822%29_.JPG | 22]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2822%29_.JPG | 22]]</td>
-
   <td></td>  
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   <td>14/07/14</td>  
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   <td></td>
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   <td>GFP and RFP resuspension and transformation, cont.</td>
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   <td></td>
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   <td>Beth Hickton, Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2823%29_.JPG | 23]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2823%29_.JPG | 23]]</td>
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   <td></td>  
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   <td>16/07/14</td>  
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   <td></td>
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   <td>Mini-prepping GFP and RFP.</td>
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   <td></td>
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   <td>Jessica Rollit, Edward Muir, Fran Penrose</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2824%29_.JPG | 24]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2824%29_.JPG | 24]]</td>
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   <td></td>  
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   <td>16/07/14</td>  
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   <td></td>
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   <td>GFP and RFP digestion/ligation to PSB1C3.</td>
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   <td></td>
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   <td>Beth Hickton, Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2825%29_.JPG | 25]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2825%29_.JPG | 25]]</td>
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   <td></td>  
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   <td>16/07/14</td>  
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   <td></td>
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   <td>Plasmid digestions.</td>
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   <td></td>
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   <td>Edward Muir, Callum Bailey, Benjamin Miller</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2826%29_.JPG | 26]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2826%29_.JPG | 26]]</td>
-
   <td></td>  
+
   <td>16/07/14</td>  
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   <td></td>
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   <td>RFP/GFP ligations.</td>
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   <td></td>
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   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2827%29_.JPG | 27]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2827%29_.JPG | 27]]</td>
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   <td></td>  
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   <td>16/07/14</td>  
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   <td></td>
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   <td>RFP/GFP ligations, cont.</td>
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   <td></td>
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   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2828%29_.JPG | 28]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2828%29_.JPG | 28]]</td>
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   <td></td>  
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   <td>17/07/14</td>  
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   <td></td>
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   <td>Transformation of GFP/RFP with terminator.</td>
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   <td></td>
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   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2829%29_.JPG | 29]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2829%29_.JPG | 29]]</td>
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   <td></td>  
+
   <td>18/07/14</td>  
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   <td></td>
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   <td>Making LB agar for promoters experiment.</td>
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   <td></td>
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   <td>Katie Pearce</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2830%29_.JPG | 30]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2830%29_.JPG | 30]]</td>
-
   <td></td>  
+
   <td>18/07/14</td>  
-
   <td></td>
+
   <td>Promoter experiment – quantities.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2831%29_.JPG | 31]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2831%29_.JPG | 31]]</td>
-
   <td></td>  
+
   <td>18/07/14</td>  
-
   <td></td>
+
   <td>Promoter experiment – quantities, cont.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2832%29_.JPG | 32]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2832%29_.JPG | 32]]</td>
-
   <td></td>  
+
   <td>18/07/14</td>  
-
   <td></td>
+
   <td>Promoter experiment – quantities, cont. 2.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2833%29_.JPG | 33]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2833%29_.JPG | 33]]</td>
-
   <td></td>  
+
   <td>21/07/14</td>  
-
   <td></td>
+
   <td>Promoter experiment preliminary timetable.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2834%29_.JPG | 34]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2834%29_.JPG | 34]]</td>
-
   <td></td>  
+
   <td>22/07/14</td>  
-
   <td></td>
+
   <td>Electrophoresis of RFP and GFP (with terminators).</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2835%29_.JPG | 35]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2835%29_.JPG | 35]]</td>
-
   <td></td>  
+
   <td>22/07/14</td>  
-
   <td></td>
+
   <td>Preparation of competent E. coli cells.</td>
-
   <td></td>
+
   <td>Beth Hickton, Peter Reader</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2836%29_.JPG | 36]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2836%29_.JPG | 36]]</td>
-
   <td></td>  
+
   <td>23/07/14</td>  
-
   <td></td>
+
   <td>GFP/RFP + terminator liquid broths mini-prepped.</td>
-
   <td></td>
+
   <td>Beth Hickton, Peter Reader, Jessica Rollit</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2837%29_.JPG | 37]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2837%29_.JPG | 37]]</td>
-
   <td></td>  
+
   <td>23/07/14</td>  
-
   <td></td>
+
   <td>Potassium Chromate preparation.</td>
-
   <td></td>
+
   <td>Peter Reader, Benjamin Miller, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2838%29_.JPG | 38]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2838%29_.JPG | 38]]</td>
-
   <td></td>  
+
   <td>23/07/14</td>  
-
   <td></td>
+
   <td>Transformation of other promoters.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2839%29_.JPG | 39]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2839%29_.JPG | 39]]</td>
-
   <td></td>  
+
   <td>24/07/14</td>  
-
   <td></td>
+
   <td>Moving GFP+T and RFP+T onto AMP backbones.</td>
-
   <td></td>
+
   <td>Edward Muir, Katie Pearce</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2840%29_.JPG | 40]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2840%29_.JPG | 40]]</td>
-
   <td></td>  
+
   <td>24/07/14</td>  
-
   <td></td>
+
   <td>Ligation of GFP+T and RFP+T onto AMP backbones.</td>
-
   <td></td>
+
   <td>Edward Muir, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2841%29_.JPG | 41]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2841%29_.JPG | 41]]</td>
-
   <td></td>  
+
   <td>24/07/14</td>  
-
   <td></td>
+
   <td>Potassium Dichromate colorimetry and spectrophotometry.</td>
-
   <td></td>
+
   <td>Beth Hickton, Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2842%29_.JPG | 42]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2842%29_.JPG | 42]]</td>
-
   <td></td>  
+
   <td>28/07/14</td>  
-
   <td></td>
+
   <td>Plating Anderson RBSs.</td>
-
   <td></td>
+
   <td>Beth Hickton, Edward Muir, Elize Hernandez</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2843%29_.JPG | 43]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2843%29_.JPG | 43]]</td>
-
   <td></td>  
+
   <td>28/07/14</td>  
-
   <td></td>
+
   <td>Mini-prep of reporters, promoters and RBSs.</td>
-
   <td></td>
+
   <td>Beth Hickton, Elize Hernandez, Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2844%29_.JPG | 44]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2844%29_.JPG | 44]]</td>
-
   <td></td>  
+
   <td>29/07/14</td>  
-
   <td></td>
+
   <td>COSHH Form created for Nitroglycerine experiments.</td>
-
   <td></td>
+
   <td>Beth Hickton, Peter Reader, Benjamin Miller</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2845%29_.JPG | 45]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2845%29_.JPG | 45]]</td>
-
   <td></td>  
+
   <td>30/07/14</td>  
-
   <td></td>
+
   <td>Cr(VI) / Ligand experimentation.</td>
-
   <td></td>
+
   <td>Peter Reader</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2846%29_.JPG | 46]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2846%29_.JPG | 46]]</td>
-
   <td></td>  
+
   <td>31/07/14</td>  
-
   <td></td>
+
   <td>Mini-prep of constructs.</td>
-
   <td></td>
+
   <td>Edward Muir, Elize Hernandez, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2847%29_.JPG | 47]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2847%29_.JPG | 47]]</td>
-
   <td></td>  
+
   <td>31/07/14</td>  
-
   <td></td>
+
   <td>Digestion and ligation of promoter/RBS combos.</td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2848%29_.JPG | 48]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2848%29_.JPG | 48]]</td>
-
   <td></td>  
+
   <td>31/07/14</td>  
-
   <td></td>
+
   <td>Oligo preparation.</td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2849%29_.JPG | 49]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2849%29_.JPG | 49]]</td>
-
   <td></td>  
+
   <td>31/07/14</td>  
-
   <td></td>
+
   <td>Ligation of promoter and RBs digestions.</td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey, Benjamin Miller</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2850%29_.JPG | 50]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2850%29_.JPG | 50]]</td>
-
   <td></td>  
+
   <td>01/08/14</td>  
-
   <td></td>
+
   <td>Digestion and Ligation of constructs 004 and 007.</td>
-
   <td></td>
+
   <td>Edward Muir, Jessica Rollit, Beth Hickton</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2851%29_.JPG | 51]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2851%29_.JPG | 51]]</td>
-
   <td></td>  
+
   <td>01/08/14</td>  
-
   <td></td>
+
   <td>Digestion and Ligation of constructs 004 and 007, cont.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2852%29_.JPG | 52]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2852%29_.JPG | 52]]</td>
-
   <td></td>  
+
   <td>05/08/14</td>  
-
   <td></td>
+
   <td>Colonies picked and mini-prepped.</td>
-
   <td></td>
+
   <td>Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2853%29_.JPG | 53]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2853%29_.JPG | 53]]</td>
-
   <td></td>  
+
   <td>05/08/14</td>  
-
   <td></td>
+
   <td>Colonies picked and mini-prepped, cont.</td>
-
   <td></td>
+
   <td>Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2854%29_.JPG | 54]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2854%29_.JPG | 54]]</td>
-
   <td></td>  
+
   <td>06/08/14</td>  
-
   <td></td>
+
   <td>Testing promoter/RBS combination efficiency.</td>
-
   <td></td>
+
   <td>Callum Bailey, Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2855%29_.JPG | 55]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2855%29_.JPG | 55]]</td>
-
   <td></td>  
+
   <td>06/08/14</td>  
-
   <td></td>
+
   <td>Testing promoter/RBS combination efficiency, cont.</td>
-
   <td></td>
+
   <td>Callum Bailey, Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2856%29_.JPG | 56]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2856%29_.JPG | 56]]</td>
-
   <td></td>  
+
   <td>07/08/14</td>  
-
   <td></td>
+
   <td>Picking colonies from promoter/RBS combination transformations. </td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2857%29_.JPG | 57]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2857%29_.JPG | 57]]</td>
-
   <td></td>  
+
   <td>07/08/14</td>  
-
   <td></td>
+
   <td>T. Cam experiment.</td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2858%29_.JPG | 58]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2858%29_.JPG | 58]]</td>
-
   <td></td>  
+
   <td>11/08/14</td>  
-
   <td></td>
+
   <td>Promoter/RBS combinations again.</td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2859%29_.JPG | 59]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2859%29_.JPG | 59]]</td>
-
   <td></td>  
+
   <td>11/08/14</td>  
-
   <td></td>
+
   <td>Promoter/RBS combinations again, cont.</td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2860%29_.JPG | 60]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2860%29_.JPG | 60]]</td>
-
   <td></td>  
+
   <td>11/08/14</td>  
-
   <td></td>
+
   <td>Inoculation of enzymes for construct 001 and 003.</td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2861%29_.JPG | 61]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2861%29_.JPG | 61]]</td>
-
   <td></td>  
+
   <td>12/08/14</td>  
-
   <td></td>
+
   <td>Inoculation of enzymes for construct 001 and 003, cont.</td>
-
   <td></td>
+
   <td>Edward Muir, Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2862%29_.JPG | 62]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2862%29_.JPG | 62]]</td>
-
   <td></td>  
+
   <td>12/08/14</td>  
-
   <td></td>
+
   <td>Transformation of a sample promoter/RBS combination.</td>
-
   <td></td>
+
   <td>Callum Bailey</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2863%29_.JPG | 63]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2863%29_.JPG | 63]]</td>
-
   <td></td>  
+
   <td>12/08/14</td>  
-
   <td></td>
+
   <td>T. Cam experiment for solvents and ether.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2864%29_.JPG | 64]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2864%29_.JPG | 64]]</td>
-
   <td></td>  
+
   <td>13/08/14</td>  
-
   <td></td>
+
   <td>Combination transformation results and construct testing.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
<tr>
<tr>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2865%29_.JPG | 65]]</td>
   <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2865%29_.JPG | 65]]</td>
-
   <td></td>  
+
   <td>13/08/14</td>  
-
   <td></td>
+
   <td>Putting oligos into GFP and RFP.</td>
-
   <td></td>
+
   <td>Edward Muir</td>
</tr>
</tr>
</table>
</table>
 +
 +
<p>These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.</p>
{{ExeterFooter}}
{{ExeterFooter}}

Latest revision as of 01:54, 18 October 2014

Exeter | ERASE

Lab Book

On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.


Page No. Date: Description: Carried out by:
1 30/06/14 Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed. Martyn Bennet, Beth Hickton
2 31/06/14 Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed. Beth Hickton, Edward Muir
3 01/07/14 List of promoters transformed, cont. Transformations were plated. Beth Hickton, Martyn Bennet
4 02/07/14 The previous day transformations were plated. Beth Hickton
5 02/07/14 Transformation of iGEM re-suspended promoters. Beth Hickton
6 02/07/14 Preparation of competent E. coli cells Beth Hickton
7 03/07/14 Promoter transformation results. Jessica Rollit, Martyn Bennet
8 03/07/14 E. coli growth rate. Edward Muir, Beth Hickton
9 04/07/14 Transformation of promoters and gene constructs. Edward Muir, Beth Hickton
10 07/07/14 Putting constructs into the iGEM vector and testing for transformation efficiency. Martyn Bennet, Edward Muir, Beth Hickton
11 08/07/14 Repeat construct addition into iGEM vector and testing transformation efficiency. Edward Muir, Beth Hickton, Martyn Bennet
12 08/07/14 Transforming constructs into Top10 and DH5. Beth Hickton, Martyn Bennet, Edward Muir
13 09/07/14 Results of Construction of iGEM vector. Jessica Rollit, Beth Hickton
14 10/07/14 A range of mini-preps Jessica Rollit, Beth Hickton
15 11/07/14 Measuring the length of DNA. Edward Muir
16 11/07/14 Measuring the length of DNA, cont. Edward Muir
17 11/07/14 Mini-prepping picked cultures containing promoters. Callum Bailey, Elize Hernandez
18 11/07/14 Parts sent for sequencing Callum Bailey, Elize Hernandez
19 11/07/14 Linearizing DNA. Edward Muir
20 11/07/14 Creating glycerol stocks. Beth Hickton, Max Smart
21 14/07/14 GFP and RFP resuspension and transformation. Edward Muir, Martyn Bennet, Beth Hickton
22 14/07/14 GFP and RFP resuspension and transformation, cont. Beth Hickton, Edward Muir
23 16/07/14 Mini-prepping GFP and RFP. Jessica Rollit, Edward Muir, Fran Penrose
24 16/07/14 GFP and RFP digestion/ligation to PSB1C3. Beth Hickton, Edward Muir
25 16/07/14 Plasmid digestions. Edward Muir, Callum Bailey, Benjamin Miller
26 16/07/14 RFP/GFP ligations. Edward Muir
27 16/07/14 RFP/GFP ligations, cont. Edward Muir
28 17/07/14 Transformation of GFP/RFP with terminator. Edward Muir
29 18/07/14 Making LB agar for promoters experiment. Katie Pearce
30 18/07/14 Promoter experiment – quantities. Edward Muir
31 18/07/14 Promoter experiment – quantities, cont. Edward Muir
32 18/07/14 Promoter experiment – quantities, cont. 2. Edward Muir
33 21/07/14 Promoter experiment preliminary timetable. Edward Muir
34 22/07/14 Electrophoresis of RFP and GFP (with terminators). Edward Muir
35 22/07/14 Preparation of competent E. coli cells. Beth Hickton, Peter Reader
36 23/07/14 GFP/RFP + terminator liquid broths mini-prepped. Beth Hickton, Peter Reader, Jessica Rollit
37 23/07/14 Potassium Chromate preparation. Peter Reader, Benjamin Miller, Beth Hickton
38 23/07/14 Transformation of other promoters. Edward Muir
39 24/07/14 Moving GFP+T and RFP+T onto AMP backbones. Edward Muir, Katie Pearce
40 24/07/14 Ligation of GFP+T and RFP+T onto AMP backbones. Edward Muir, Beth Hickton
41 24/07/14 Potassium Dichromate colorimetry and spectrophotometry. Beth Hickton, Edward Muir
42 28/07/14 Plating Anderson RBSs. Beth Hickton, Edward Muir, Elize Hernandez
43 28/07/14 Mini-prep of reporters, promoters and RBSs. Beth Hickton, Elize Hernandez, Edward Muir
44 29/07/14 COSHH Form created for Nitroglycerine experiments. Beth Hickton, Peter Reader, Benjamin Miller
45 30/07/14 Cr(VI) / Ligand experimentation. Peter Reader
46 31/07/14 Mini-prep of constructs. Edward Muir, Elize Hernandez, Beth Hickton
47 31/07/14 Digestion and ligation of promoter/RBS combos. Edward Muir, Callum Bailey
48 31/07/14 Oligo preparation. Edward Muir, Callum Bailey
49 31/07/14 Ligation of promoter and RBs digestions. Edward Muir, Callum Bailey, Benjamin Miller
50 01/08/14 Digestion and Ligation of constructs 004 and 007. Edward Muir, Jessica Rollit, Beth Hickton
51 01/08/14 Digestion and Ligation of constructs 004 and 007, cont. Edward Muir
52 05/08/14 Colonies picked and mini-prepped. Callum Bailey
53 05/08/14 Colonies picked and mini-prepped, cont. Callum Bailey
54 06/08/14 Testing promoter/RBS combination efficiency. Callum Bailey, Edward Muir
55 06/08/14 Testing promoter/RBS combination efficiency, cont. Callum Bailey, Edward Muir
56 07/08/14 Picking colonies from promoter/RBS combination transformations. Edward Muir, Callum Bailey
57 07/08/14 T. Cam experiment. Edward Muir, Callum Bailey
58 11/08/14 Promoter/RBS combinations again. Edward Muir, Callum Bailey
59 11/08/14 Promoter/RBS combinations again, cont. Edward Muir, Callum Bailey
60 11/08/14 Inoculation of enzymes for construct 001 and 003. Edward Muir, Callum Bailey
61 12/08/14 Inoculation of enzymes for construct 001 and 003, cont. Edward Muir, Callum Bailey
62 12/08/14 Transformation of a sample promoter/RBS combination. Callum Bailey
63 12/08/14 T. Cam experiment for solvents and ether. Edward Muir
64 13/08/14 Combination transformation results and construct testing. Edward Muir
65 13/08/14 Putting oligos into GFP and RFP. Edward Muir

These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.

Exeter | ERASE

Retrieved from "http://2014.igem.org/Team:Exeter/LabBook"