Team:Paris Saclay/Notebook/July/31

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{{Team:Paris_Saclay/notebook_header}}
=Thursday 31st July=
=Thursday 31st July=
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==Lab work==
==Lab work==
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===C - Salicylate Inducible Suppressing System===
===C - Salicylate Inducible Suppressing System===
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====Transformation in DH5α====
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====Transformation of competent E.coli cells====
''by Fabio''
''by Fabio''
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We transformed in DH5α the Ligation's result made [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System yesterday] ('''BBa_J61051''' and '''BBa_K228001''').
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We transformed in DH5α the Ligation's result made [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System yesterday] ('''BBa_J61051''' with '''BBa_K228001''').
'''Samples:'''
'''Samples:'''
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# Sample 2 μl
# Sample 2 μl
# Sample 1 μl
# Sample 1 μl
# DH5α Control
# DH5α Control
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'''Results on 1st August morning:'''
'''Results on 1st August morning:'''
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# Success, grown very well (± 100 colonies)
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# Success, grown very well (+- 100 colonies)
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# Success, grown well (± 50 colonies)
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# Success, grown well (+- 50 colonies)
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# Success, nothing has grown
# Success, nothing has grown
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
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Reunion, Handled topics:
Reunion, Handled topics:
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* Organisation of the Meeting with french teams
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* Organisation of the Meeting with French teams
* Great discussion about Human Practices
* Great discussion about Human Practices
* Discussion about the logo
* Discussion about the logo
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==Photo of the Day==
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[[File:Paris Saclay 31_july.jpg|600px|center]]
'''Members present:'''
'''Members present:'''
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* Students: Arnaud, Fabio, Romain, Sean and Terry.
* Students: Arnaud, Fabio, Romain, Sean and Terry.
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 15:45, 14 October 2014

Contents

Thursday 31st July

Lab work

C - Salicylate Inducible Suppressing System

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α the Ligation's result made yesterday (BBa_J61051 with BBa_K228001).

Samples:

  1. Sample 2 μl
  2. Sample 1 μl
  3. DH5α Control

Results on 1st August morning:

  1. Success, grown very well (± 100 colonies)
  2. Success, grown well (± 50 colonies)
  3. Success, nothing has grown

Protocol

D - Lemon scent

by Romain

2 Precultures of DY330 with pJBEI-6409 in 2ml LB and Cm.

Human Pratices

Reunion, Handled topics:

  • Organisation of the Meeting with French teams
  • Great discussion about Human Practices
  • Discussion about the logo


Photo of the Day

Paris Saclay 31 july.jpg

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Romain, Sean and Terry.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/31"