Team:Paris Saclay/Notebook/July/17

From 2014.igem.org

(Difference between revisions)
(3 - PCR and Electrophoresis)
m
 
(6 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Paris_Saclay/notebook_header}}
=Thursday 17th July=
=Thursday 17th July=
-
 
==Lab work==
==Lab work==
-
 
+
===The chassis coli Odor free===
-
===1 - Preparation of supercompetent cells===
+
====1 - Preparation of supercompetent cells====
-
 
+
''By Mathias & Romain''
''By Mathias & Romain''
Line 11: Line 10:
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
-
===2 - Bacterial Culture===
+
====2 - Bacterial Culture====
-
 
+
''by Fabio and Maher''
''by Fabio and Maher''
2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 16th July]
2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 16th July]
-
===3 - PCR and Electrophoresis===
+
====3 - PCR and Electrophoresis====
-
 
+
[[File:Electrophoresis_17.07.2014.jpg|400px|right]]
[[File:Electrophoresis_17.07.2014.jpg|400px|right]]
''by Arnaud & Mathias''
''by Arnaud & Mathias''
Line 34: Line 31:
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
 +
 +
==Modeling==
 +
====Study of bacterial population growth in space and time====
 +
[[File:Book.jpg|100px|left]]
 +
''by Pierre & Juliette''
 +
 +
We have finished to study a first model for our problem. It is a simple birth/death model and we do a mix of deterministic and stochastic model in order to be most precise as possible.
 +
 +
We have focused our study on this (excellent !) book :  ''Modeling Biological Populations in Space and Time''
 +
by '''Eric Renshaw''', and especially on chapters 1, 2 and 3.
 +
 +
We now hope to succeed improving our model to be more realistic !
 +
 +
 +
==Photo of the Day==
 +
[[File:Paris Saclay 17_july.jpg|600px|center]]
'''Members present:'''
'''Members present:'''
* Instructors and advisors: Alice and Solenne.
* Instructors and advisors: Alice and Solenne.
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
-
 
+
{{Team:Paris_Saclay/notebook_footer}}
-
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
+

Latest revision as of 13:57, 17 October 2014

Contents

Thursday 17th July

Lab work

The chassis coli Odor free

1 - Preparation of supercompetent cells

By Mathias & Romain

Strains used: MG1655 (x2), MG1655Z1 (x2).

Protocol

2 - Bacterial Culture

by Fabio and Maher

2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the 16th July

3 - PCR and Electrophoresis

Electrophoresis 17.07.2014.jpg

by Arnaud & Mathias

PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine).

  1. Molecular weight marker 1kb
  2. Empty
  3. IPS70 and IPS71 (1)
  4. IPS70 and IPS71 (2)

Results:

Failure, no PCR products.

Protocol

Modeling

Study of bacterial population growth in space and time

Book.jpg

by Pierre & Juliette

We have finished to study a first model for our problem. It is a simple birth/death model and we do a mix of deterministic and stochastic model in order to be most precise as possible.

We have focused our study on this (excellent !) book : Modeling Biological Populations in Space and Time by Eric Renshaw, and especially on chapters 1, 2 and 3.

We now hope to succeed improving our model to be more realistic !


Photo of the Day

Paris Saclay 17 july.jpg

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar