Latest revision as of 15:45, 14 October 2014

Thursday 31st July

by Fabio

We transformed in DH5α the Ligation's result made yesterday (BBa_J61051 with BBa_K228001).

Samples:

Results on 1st August morning:

by Romain

2 Precultures of DY330 with pJBEI-6409 in 2ml LB and Cm.

Reunion, Handled topics:

Members present:

@@ Line 1: / Line 1: @@
+{{Team:Paris_Saclay/notebook_header}}
 =Thursday 31st July=
 ==Lab work==
 ===C - Salicylate Inducible Suppressing System===
-====Transformation in DH5 alpha====
+====Transformation of competent E.coli cells====
-''In progress''
+''by Fabio''
-====Bacterial Culture====
+We transformed in DH5α the Ligation's result made [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System yesterday] ('''BBa_J61051''' with '''BBa_K228001''').
-''In progress''
+'''Samples:'''
+# Sample 2 μl
+# Sample 1 μl
+# DH5α Control
+'''Results on 1st August morning:'''
+# Success, grown very well (± 100 colonies)
+# Success, grown well (± 50 colonies)
+# Success, nothing has grown
+[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
 ===D - Lemon scent===
@@ Line 17: / Line 25: @@
 ==Human Pratices==
+Reunion, Handled topics:
+* Organisation of the Meeting with French teams
+* Great discussion about Human Practices
+* Discussion about the logo
+==Photo of the Day==
+[[File:Paris Saclay 31_july.jpg|600px|center]]
 '''Members present:'''
@@ Line 22: / Line 38: @@
 * Students: Arnaud, Fabio, Romain, Sean and Terry.
-[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
+{{Team:Paris_Saclay/notebook_footer}}