Team:Paris Saclay/Protocols/PCR clean-up
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- | = | + | {{Team:Paris_Saclay/protocols_header}} |
+ | =PCR clean-up= | ||
- | After the PCR, pool your PCR result in an eppendorf 1,5ml. | + | # After the PCR, pool your PCR result in an eppendorf 1,5ml. |
- | Add | + | # Add double the amount of PCR result in NTI. |
- | + | # Centrifuge at 11000g, 30 seconds RT. | |
- | Centrifuge at 11000g, 30 | + | # Discard the supernatant, wash a first time with 700µl NT3. |
- | + | # Centrifuge at 11000g, 30 secondes RT. | |
- | Discard the supernatant, wash first time with 700µl NT3. | + | # Discard the supernatant, wash a second time with 700µl NT3. |
- | + | # Centrifuge at 11000g, 30 seconds RT. | |
- | Centrifuge at 11000g, 30 secondes RT. | + | # Centrifuge the column empty. |
- | + | # Add 15-30µl elution buffer and centrifuge at 11000g, 1 min RT. | |
- | Discard the supernatant, wash a second time with 700µl NT3. | + | # Collect your DNA in an eppendorf 1,5ml. |
- | + | {{Team:Paris_Saclay/protocols_footer}} | |
- | Centrifuge at 11000g, 30 | + | |
- | + | ||
- | Centrifuge the column empty. | + | |
- | + | ||
- | Add | + | |
- | + | ||
- | Collect your DNA in an eppendorf 1,5ml. | + |
Latest revision as of 11:53, 11 August 2014
PCR clean-up
- After the PCR, pool your PCR result in an eppendorf 1,5ml.
- Add double the amount of PCR result in NTI.
- Centrifuge at 11000g, 30 seconds RT.
- Discard the supernatant, wash a first time with 700µl NT3.
- Centrifuge at 11000g, 30 secondes RT.
- Discard the supernatant, wash a second time with 700µl NT3.
- Centrifuge at 11000g, 30 seconds RT.
- Centrifuge the column empty.
- Add 15-30µl elution buffer and centrifuge at 11000g, 1 min RT.
- Collect your DNA in an eppendorf 1,5ml.