"
Team:Paris Saclay/Notebook/June/30
From 2014.igem.org
(Difference between revisions)
(→2 - Réhydration of selected biobricks:) |
m (→Photo of the Day) |
||
| (11 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{Team:Paris_Saclay/notebook_header}} | ||
=Monday 30th June= | =Monday 30th June= | ||
| - | |||
==Lab work== | ==Lab work== | ||
| - | + | === The odor-free ''E. coli'' chassis=== | |
| - | ==== | + | ====Culture of the supercompetents==== |
| - | + | ||
''by Mathias'' | ''by Mathias'' | ||
| Line 12: | Line 11: | ||
1ml of an overnight culture in 100ml of LB. | 1ml of an overnight culture in 100ml of LB. | ||
| - | ==== | + | ====Rehydration of selected biobricks==== |
| - | + | ||
''by Juliette'' | ''by Juliette'' | ||
| - | * BBa_I9026 | + | * BBa_I9026 (rhII translational unit) |
| - | * BBa_K592025 | + | * BBa_K592025 (BB0034-amilCP) |
| - | * BBa_K786002 | + | * BBa_K786002 (sensory rhodopsin II & Tar sensitive to blue-green light) |
| - | * BBa_K786003 | + | * BBa_K786003 (sensory rhodopsin I & Tar sensitive to orange light) |
| - | * BBa_K778002 | + | * BBa_K778002 |
| - | * BBa_K592011 | + | * BBa_K592011 (cjBlue green chromoprotein)(2 times) |
| - | * BBa_K592009 | + | * BBa_K592009 (amilCP blue chromoprotein) |
| - | * | + | * BBa_K103391 (Yellow chromoprotein and Lime yellow-green chromoprotein)(2 times) |
| - | * BBa_J45014 | + | * BBa_J45014 (lemon scent, alcohol acetyltransferase I) |
| - | * BBa_K517003 | + | * BBa_K517003 (lemon scent, β-pinene) |
| - | + | ||
| - | + | ||
| + | ====Transformations==== | ||
''by Maher & Mathias'' | ''by Maher & Mathias'' | ||
| - | We use the DH5α provided by our advisors for the aforementioned Biobricks. | + | We use the ''E. coli'' DH5α supercompetent cells provided by our advisors for the aforementioned Biobricks. |
Spread on petri dishes with the concentration 10<sup>-1</sup> and 10<sup>-2</sup>. | Spread on petri dishes with the concentration 10<sup>-1</sup> and 10<sup>-2</sup>. | ||
| - | ==== | + | ====Preparations==== |
| + | ''by Marie and Mathieu'' | ||
| - | * Aliquots of antibiotics | + | * Aliquots of antibiotics |
| - | + | ||
| - | + | ||
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. | Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. | ||
(Do the same for Kanamycin and Ampicillin) | (Do the same for Kanamycin and Ampicillin) | ||
| - | |||
| - | |||
''by Romain'' | ''by Romain'' | ||
| - | + | * Petri dishes (with antiobiotics) | |
| - | + | Add 250µl of antibiotics in 250ml of LAG. Pour in Petri dishes. | |
''by Arnaud'' | ''by Arnaud'' | ||
| + | |||
| + | * Range of gelose with different concentration of agar | ||
Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water. | Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water. | ||
| + | |||
| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 30_june.jpg|400px|center]] | ||
'''Members present:''' | '''Members present:''' | ||
| - | |||
* Instructors : Solenne. | * Instructors : Solenne. | ||
* Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain. | * Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain. | ||
| - | + | {{Team:Paris_Saclay/notebook_footer}} | |
| - | + | ||
Latest revision as of 15:46, 14 October 2014
Contents |
Monday 30th June
Lab work
The odor-free E. coli chassis
Culture of the supercompetents
by Mathias
- MG1655
- MG1655Z1
1ml of an overnight culture in 100ml of LB.
Rehydration of selected biobricks
by Juliette
- BBa_I9026 (rhII translational unit)
- BBa_K592025 (BB0034-amilCP)
- BBa_K786002 (sensory rhodopsin II & Tar sensitive to blue-green light)
- BBa_K786003 (sensory rhodopsin I & Tar sensitive to orange light)
- BBa_K778002
- BBa_K592011 (cjBlue green chromoprotein)(2 times)
- BBa_K592009 (amilCP blue chromoprotein)
- BBa_K103391 (Yellow chromoprotein and Lime yellow-green chromoprotein)(2 times)
- BBa_J45014 (lemon scent, alcohol acetyltransferase I)
- BBa_K517003 (lemon scent, β-pinene)
Transformations
by Maher & Mathias
We use the E. coli DH5α supercompetent cells provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10-1 and 10-2.
Preparations
by Marie and Mathieu
- Aliquots of antibiotics
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)
by Romain
- Petri dishes (with antiobiotics)
Add 250µl of antibiotics in 250ml of LAG. Pour in Petri dishes.
by Arnaud
- Range of gelose with different concentration of agar
Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.
Photo of the Day
Members present:
- Instructors : Solenne.
- Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
