Latest revision as of 15:41, 14 October 2014

Wednesday 23rd July

Lab work

The Suppressing Salicylate System

Rehydration of BioBricks

by Fabio

The first step of this Subproject is to rehydrate the following BioBricks. Both provided in the iGEM 2014 DNA Kit Distribution.

BBa_J61051 - Salicylate promoter + NahR
BBa_K228001 - RNA suppressor

Lemon scent

p cola part 2

by Sean

p cola DNA was extracted in several instances yesterday. Today we attempt to collect all of the DNA and precipitate it.

Protocol

Add equal parts plasmid DNA and ethanol 100% in a 1.5 microcentrifuge tube.
If total volume is V, then add V/5 of sodium acetate (initial concentration of NaAc is 3M; we want a final concentration of .3M). Numerical application: Total volume of p cola was 150μl. Hence 150 μl of ethanol 100% was added, as well as 30μl of NaAc 3M. Incubate at -20°C for 30 minutes. (5:20pm - 5:50pm in our case)
Centrifuge for 10 minutes at 4°C and at 11000g.
Discard supernatant.
Add 1ml of ethanol 70%.
Repeat step 3.
Discard supernatant. Make tube interior as dry as possible. Tip: use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. Note: we decided to leave the tube at room temperature and continue tomorrow instead.
(tomorrow) Resuspend pellet in 30μl H₂O milliQ.

Modeling

Meeting of modeling with advisor Claire to talk about:

Diffusion of O₂ in the gel
Bacterial growth on the surface
translecture system
Diffusion of the limonene

Reunion

Handled topics:

Collaborations with Colombia, Paris Bettencourt and Virginia
Organisation of the Meeting with french teams
Definitions of the sub projects
Definitions of notebook's standards
Organisation of the Wiki
Great discussion about Human Practices

Photo of the Day

Members there:

Instructors and advisors: Solenne and Sylvie.
Students: Arnaud, Eugene, Fabio, Juliette, Leila, Pierre, Romain, Sean and Terry.

Back to the calendar

@@ Line 1: / Line 1: @@
+{{Team:Paris_Saclay/notebook_header}}
 =Wednesday 23rd July=
 ==Lab work==
+=== The Suppressing Salicylate System===
+====Rehydration of BioBricks====
+''by Fabio''
-===1 - p cola part 2===
+The first step of this Subproject is to rehydrate the following BioBricks. Both provided in the iGEM 2014 DNA Kit Distribution.
+* BBa_J61051 - ''Salicylate promoter + NahR''
+* BBa_K228001 - ''RNA suppressor''
+===Lemon scent===
+====p cola part 2====
 ''by Sean''
@@ Line 17: / Line 26: @@
 # Discard supernatant. Make tube interior as dry as possible. '''Tip''': use a paper towel on which to lightly tap the rim of the tube. Dry pellet in oven at 37°C. <span style="color:red">Note: we decided to leave the tube at room temperature and continue tomorrow instead.</span>
 # <font color="#666666">(tomorrow) Resuspend pellet in 30μl H<sub>2</sub>O milliQ.</font>
+==Modeling==
+Meeting of modeling with advisor Claire to talk about:
+* Diffusion of O<sub>2</sub> in the gel
+* Bacterial growth on the surface
+* translecture system
+* Diffusion of the limonene
 ==Reunion==
+Handled topics:
+* Collaborations with Colombia, Paris Bettencourt and Virginia
+* Organisation of the Meeting with french teams
+* Definitions of the sub projects
+* Definitions of notebook's standards
+* Organisation of the Wiki
+* Great discussion about Human Practices
-We talk about the organisation of the wiki's notebook, the different parts of our project and the ethic points.
+==Photo of the Day==
+[[File:Paris Saclay 23_july.jpg|600px|center]]
 '''Members there''':
 * Instructors and advisors: Solenne and Sylvie.
 * Students: Arnaud, Eugene, Fabio, Juliette, Leila, Pierre, Romain, Sean and Terry.
+{{Team:Paris_Saclay/notebook_footer}}
-[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Team:Paris Saclay/Notebook/July/23

From 2014.igem.org