Latest revision as of 13:57, 17 October 2014

Thursday 17th July

Lab work

The chassis coli Odor free

1 - Preparation of supercompetent cells

By Mathias & Romain

Strains used: MG1655 (x2), MG1655Z1 (x2).

Protocol

2 - Bacterial Culture

by Fabio and Maher

2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the 16th July

3 - PCR and Electrophoresis

by Arnaud & Mathias

PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine).

Molecular weight marker 1kb
Empty
IPS70 and IPS71 (1)
IPS70 and IPS71 (2)

Results:

Failure, no PCR products.

Protocol

Modeling

Study of bacterial population growth in space and time

by Pierre & Juliette

We have finished to study a first model for our problem. It is a simple birth/death model and we do a mix of deterministic and stochastic model in order to be most precise as possible.

We have focused our study on this (excellent !) book : Modeling Biological Populations in Space and Time by Eric Renshaw, and especially on chapters 1, 2 and 3.

We now hope to succeed improving our model to be more realistic !

Photo of the Day

Members present:

Instructors and advisors: Alice and Solenne.
Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar

@@ Line 1: / Line 1: @@
+{{Team:Paris_Saclay/notebook_header}}
 =Thursday 17th July=
 ==Lab work==
+===The chassis coli Odor free===
+====1 - Preparation of supercompetent cells====
+''By Mathias & Romain''
-====1 - bacterial culture & OD====
+Strains used: MG1655 (x2), MG1655Z1 (x2).
-''By Mathias, Romain & Sean''
+[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
-Strains used: MG1655, MG1655Z1.
+====2 - Bacterial Culture====
+''by Fabio and Maher''
-[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
+liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 16th July]
+====3 - PCR and Electrophoresis====
+[[File:Electrophoresis_17.07.2014.jpg|400px|right]]
+''by Arnaud & Mathias''
+PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine).
+#Molecular weight marker 1kb
+#Empty
+#IPS70 and IPS71 (1)
+#IPS70 and IPS71 (2)
+'''Results:'''
+Failure, no PCR products.
+[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
+==Modeling==
+====Study of bacterial population growth in space and time====
+[[File:Book.jpg|100px|left]]
+''by Pierre & Juliette''
+We have finished to study a first model for our problem. It is a simple birth/death model and we do a mix of deterministic and stochastic model in order to be most precise as possible.
+We have focused our study on this (excellent !) book :  ''Modeling Biological Populations in Space and Time''
+by '''Eric Renshaw''', and especially on chapters 1, 2 and 3.
+We now hope to succeed improving our model to be more realistic !
+==Photo of the Day==
+[[File:Paris Saclay 17_july.jpg|600px|center]]
-[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
+'''Members present:'''
+* Instructors and advisors: Alice and Solenne.
+* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
+{{Team:Paris_Saclay/notebook_footer}}

Team:Paris Saclay/Notebook/July/17

From 2014.igem.org