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Team:Paris Saclay/Notebook/July/16
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=Wednesday 16th July= | =Wednesday 16th July= | ||
| - | |||
==Lab work== | ==Lab work== | ||
| - | + | ===Transformation of competent E.coli cells=== | |
| - | === | + | |
| - | + | ||
''by Fabio, Maher and Romain'' | ''by Fabio, Maher and Romain'' | ||
We transformed in DH5α the following Biobrick: | We transformed in DH5α the following Biobrick: | ||
| - | * | + | * BBa_J23119 |
'''Samples:''' | '''Samples:''' | ||
| - | |||
# Concentrated | # Concentrated | ||
# Undiluted | # Undiluted | ||
| - | # Diluted 10 | + | # Diluted 10<sup>-1</sup> |
# Control | # Control | ||
'''Results:''' | '''Results:''' | ||
| - | |||
# Concentrated: 99 colonies | # Concentrated: 99 colonies | ||
# Undiluted: 5 colonies | # Undiluted: 5 colonies | ||
| Line 26: | Line 22: | ||
'''Conclusion:''' | '''Conclusion:''' | ||
| - | |||
Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures. | Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures. | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
| + | |||
| + | ===2 - Plasmid DNA Purification=== | ||
| + | ''by Mathieu'' | ||
| + | |||
| + | *BBa_J23100 (x2) | ||
| + | *BBa_J23106 (x2) | ||
| + | *BBa_J23114 (x2) | ||
| + | |||
| + | from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/15 15th July] | ||
| + | |||
| + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | ||
==Reunion== | ==Reunion== | ||
| - | |||
Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning) | Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning) | ||
| Line 40: | Line 45: | ||
''Arnaud represents us'' | ''Arnaud represents us'' | ||
| - | [ | + | ==Photo of the Day== |
| + | [[File:Paris Saclay 16_july.jpg|600px|center]] | ||
| + | |||
| + | '''Members present:''' | ||
| + | * Instructors and advisors: Alice, Solenne and Sylvie. | ||
| + | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
| + | |||
| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 13:45, 14 October 2014
Contents |
Wednesday 16th July
Lab work
Transformation of competent E.coli cells
by Fabio, Maher and Romain
We transformed in DH5α the following Biobrick:
- BBa_J23119
Samples:
- Concentrated
- Undiluted
- Diluted 10-1
- Control
Results:
- Concentrated: 99 colonies
- Undiluted: 5 colonies
- Diluted: 0
- Control: 0
Conclusion: Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.
2 - Plasmid DNA Purification
by Mathieu
- BBa_J23100 (x2)
- BBa_J23106 (x2)
- BBa_J23114 (x2)
from Bacterial Culture made the 15th July
Reunion
Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)
Reunion: Lab meeting to discuss the project. (afternoon)
Meeting with Evry's Team. (night) Arnaud represents us
Photo of the Day
Members present:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
