Team:Paris Saclay/Notebook/July/17

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{{Team:Paris_Saclay/notebook_header}}
=Thursday 17th July=
=Thursday 17th July=
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==Lab work==
==Lab work==
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===The chassis coli Odor free===
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====1 - Preparation of supercompetent cells====
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''By Mathias & Romain''
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Strains used: MG1655 (x2), MG1655Z1 (x2).
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
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====2 - Bacterial Culture====
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''by Fabio and Maher''
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2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 16th July]
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====3 - PCR and Electrophoresis====
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[[File:Electrophoresis_17.07.2014.jpg|400px|right]]
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''by Arnaud & Mathias''
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PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine).
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#Molecular weight marker 1kb
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#Empty
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#IPS70 and IPS71 (1)
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#IPS70 and IPS71 (2)
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'''Results:'''
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Failure, no PCR products.
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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==Modeling==
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====Study of bacterial population growth in space and time====
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[[File:Book.jpg|100px|left]]
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''by Pierre & Juliette''
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We have finished to study a first model for our problem. It is a simple birth/death model and we do a mix of deterministic and stochastic model in order to be most precise as possible.
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We have focused our study on this (excellent !) book :  ''Modeling Biological Populations in Space and Time''
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by '''Eric Renshaw''', and especially on chapters 1, 2 and 3.
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We now hope to succeed improving our model to be more realistic !
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==Photo of the Day==
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[[File:Paris Saclay 17_july.jpg|600px|center]]
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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'''Members present:'''
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* Instructors and advisors: Alice and Solenne.
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* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 13:57, 17 October 2014

Contents

Thursday 17th July

Lab work

The chassis coli Odor free

1 - Preparation of supercompetent cells

By Mathias & Romain

Strains used: MG1655 (x2), MG1655Z1 (x2).

Protocol

2 - Bacterial Culture

by Fabio and Maher

2 liquid cultures with 5ml of LB and 5µl of amp (at 9am - 37°C - 150 rpm), both BBa_J23119 from the concentrated colony made the 16th July

3 - PCR and Electrophoresis

Electrophoresis 17.07.2014.jpg

by Arnaud & Mathias

PCR targeting with oligos IPS70 and IPS71 of pOSV230 (Apramycine).

  1. Molecular weight marker 1kb
  2. Empty
  3. IPS70 and IPS71 (1)
  4. IPS70 and IPS71 (2)

Results:

Failure, no PCR products.

Protocol

Modeling

Study of bacterial population growth in space and time

Book.jpg

by Pierre & Juliette

We have finished to study a first model for our problem. It is a simple birth/death model and we do a mix of deterministic and stochastic model in order to be most precise as possible.

We have focused our study on this (excellent !) book : Modeling Biological Populations in Space and Time by Eric Renshaw, and especially on chapters 1, 2 and 3.

We now hope to succeed improving our model to be more realistic !


Photo of the Day

Paris Saclay 17 july.jpg

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/17"