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Team:Exeter/LabBook
From 2014.igem.org
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<td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%282%29_.JPG | 2]]</td> | <td>[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%282%29_.JPG | 2]]</td> | ||
<td>31/06/14</td> | <td>31/06/14</td> | ||
| - | <td>Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed./td> | + | <td>Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed.</td> |
<td>Beth Hickton, Edward Muir</td> | <td>Beth Hickton, Edward Muir</td> | ||
</tr> | </tr> | ||
Latest revision as of 01:54, 18 October 2014
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Lab Book
On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.
Page No. Date: Description: Carried out by: 1 30/06/14 Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed. Martyn Bennet, Beth Hickton 2 31/06/14 Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed. Beth Hickton, Edward Muir 3 01/07/14 List of promoters transformed, cont. Transformations were plated. Beth Hickton, Martyn Bennet 4 02/07/14 The previous day transformations were plated. Beth Hickton 5 02/07/14 Transformation of iGEM re-suspended promoters. Beth Hickton 6 02/07/14 Preparation of competent E. coli cells Beth Hickton 7 03/07/14 Promoter transformation results. Jessica Rollit, Martyn Bennet 8 03/07/14 E. coli growth rate. Edward Muir, Beth Hickton 9 04/07/14 Transformation of promoters and gene constructs. Edward Muir, Beth Hickton 10 07/07/14 Putting constructs into the iGEM vector and testing for transformation efficiency. Martyn Bennet, Edward Muir, Beth Hickton 11 08/07/14 Repeat construct addition into iGEM vector and testing transformation efficiency. Edward Muir, Beth Hickton, Martyn Bennet 12 08/07/14 Transforming constructs into Top10 and DH5. Beth Hickton, Martyn Bennet, Edward Muir 13 09/07/14 Results of Construction of iGEM vector. Jessica Rollit, Beth Hickton 14 10/07/14 A range of mini-preps Jessica Rollit, Beth Hickton 15 11/07/14 Measuring the length of DNA. Edward Muir 16 11/07/14 Measuring the length of DNA, cont. Edward Muir 17 11/07/14 Mini-prepping picked cultures containing promoters. Callum Bailey, Elize Hernandez 18 11/07/14 Parts sent for sequencing Callum Bailey, Elize Hernandez 19 11/07/14 Linearizing DNA. Edward Muir 20 11/07/14 Creating glycerol stocks. Beth Hickton, Max Smart 21 14/07/14 GFP and RFP resuspension and transformation. Edward Muir, Martyn Bennet, Beth Hickton 22 14/07/14 GFP and RFP resuspension and transformation, cont. Beth Hickton, Edward Muir 23 16/07/14 Mini-prepping GFP and RFP. Jessica Rollit, Edward Muir, Fran Penrose 24 16/07/14 GFP and RFP digestion/ligation to PSB1C3. Beth Hickton, Edward Muir 25 16/07/14 Plasmid digestions. Edward Muir, Callum Bailey, Benjamin Miller 26 16/07/14 RFP/GFP ligations. Edward Muir 27 16/07/14 RFP/GFP ligations, cont. Edward Muir 28 17/07/14 Transformation of GFP/RFP with terminator. Edward Muir 29 18/07/14 Making LB agar for promoters experiment. Katie Pearce 30 18/07/14 Promoter experiment – quantities. Edward Muir 31 18/07/14 Promoter experiment – quantities, cont. Edward Muir 32 18/07/14 Promoter experiment – quantities, cont. 2. Edward Muir 33 21/07/14 Promoter experiment preliminary timetable. Edward Muir 34 22/07/14 Electrophoresis of RFP and GFP (with terminators). Edward Muir 35 22/07/14 Preparation of competent E. coli cells. Beth Hickton, Peter Reader 36 23/07/14 GFP/RFP + terminator liquid broths mini-prepped. Beth Hickton, Peter Reader, Jessica Rollit 37 23/07/14 Potassium Chromate preparation. Peter Reader, Benjamin Miller, Beth Hickton 38 23/07/14 Transformation of other promoters. Edward Muir 39 24/07/14 Moving GFP+T and RFP+T onto AMP backbones. Edward Muir, Katie Pearce 40 24/07/14 Ligation of GFP+T and RFP+T onto AMP backbones. Edward Muir, Beth Hickton 41 24/07/14 Potassium Dichromate colorimetry and spectrophotometry. Beth Hickton, Edward Muir 42 28/07/14 Plating Anderson RBSs. Beth Hickton, Edward Muir, Elize Hernandez 43 28/07/14 Mini-prep of reporters, promoters and RBSs. Beth Hickton, Elize Hernandez, Edward Muir 44 29/07/14 COSHH Form created for Nitroglycerine experiments. Beth Hickton, Peter Reader, Benjamin Miller 45 30/07/14 Cr(VI) / Ligand experimentation. Peter Reader 46 31/07/14 Mini-prep of constructs. Edward Muir, Elize Hernandez, Beth Hickton 47 31/07/14 Digestion and ligation of promoter/RBS combos. Edward Muir, Callum Bailey 48 31/07/14 Oligo preparation. Edward Muir, Callum Bailey 49 31/07/14 Ligation of promoter and RBs digestions. Edward Muir, Callum Bailey, Benjamin Miller 50 01/08/14 Digestion and Ligation of constructs 004 and 007. Edward Muir, Jessica Rollit, Beth Hickton 51 01/08/14 Digestion and Ligation of constructs 004 and 007, cont. Edward Muir 52 05/08/14 Colonies picked and mini-prepped. Callum Bailey 53 05/08/14 Colonies picked and mini-prepped, cont. Callum Bailey 54 06/08/14 Testing promoter/RBS combination efficiency. Callum Bailey, Edward Muir 55 06/08/14 Testing promoter/RBS combination efficiency, cont. Callum Bailey, Edward Muir 56 07/08/14 Picking colonies from promoter/RBS combination transformations. Edward Muir, Callum Bailey 57 07/08/14 T. Cam experiment. Edward Muir, Callum Bailey 58 11/08/14 Promoter/RBS combinations again. Edward Muir, Callum Bailey 59 11/08/14 Promoter/RBS combinations again, cont. Edward Muir, Callum Bailey 60 11/08/14 Inoculation of enzymes for construct 001 and 003. Edward Muir, Callum Bailey 61 12/08/14 Inoculation of enzymes for construct 001 and 003, cont. Edward Muir, Callum Bailey 62 12/08/14 Transformation of a sample promoter/RBS combination. Callum Bailey 63 12/08/14 T. Cam experiment for solvents and ether. Edward Muir 64 13/08/14 Combination transformation results and construct testing. Edward Muir 65 13/08/14 Putting oligos into GFP and RFP. Edward Muir These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.
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