Team:Paris Saclay/Notebook/July/11

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{{Team:Paris_Saclay/notebook_header}}
=Friday 11th July=
=Friday 11th July=
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==Lab work==
==Lab work==
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====Fill in the WIKI (Notebook and Protocols)====
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''by Juliette & Romain''
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====1 - Fill in the WIKI (Notebook and Protocols)====
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===The chassis coli Odor free===
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====Preparation of competent cells E.coli====
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''Juliette & Romain''
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''by Maher and Sean''
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====2 - Transformation of competent cells E.coli====
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''Fabio & Maher''
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Competent strains used:  
Competent strains used:  
*DH5α
*DH5α
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*MG1655
 
*MG1655Z1
*MG1655Z1
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*DH5α+ (given by Solenne)
 
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(The strains DH5α, MG1655 and MG1655Z1 were prepared by ''Mathias & Mathieu'')
 
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BioBricks used:
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Sean did the first strain, Maher did the second one.
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*J23100 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
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*J23106 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
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*J23114 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
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+ pET28a (Kan R)(Dilutions: 0, 10^-1 and 10^-2)
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(pET28a is a plasmid used to verify that the 4 strains are competent).
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The negative controles:
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
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*pET28a: without plasmid, nothing grows.
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*J23100, J23106, J23114: without the BioBrick, nothing brows.
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Results:
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====Electrophoresis====
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*pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
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[[File:Electrophoresis_11.07.2014.jpg|400px|right]]
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*J23100, J23106, J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
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''by Fabio and Marie''
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
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#Cm FNR-RBS-AmilCP term (1)
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#Cm FNR-RBS-AmilCP term (2)
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#B0015 (1)
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#B0015 (2)
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#PCola-GES 10^-2 (1)
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#PCola-GES 10^-2 (1)
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#PCola-GES 10^-2 (2)
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#PCola-GES 10^-2 (2)
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#PJBG-6409 10^-2 (1)
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#PJBG-6409 10^-2 (2)
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#PJBG-6409 10^-2 (2)
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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==Photo of the Day==
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[[File:Paris Saclay 11_july.jpg|600px|center]]
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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'''Members present:'''
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* Instructors and advisors: Alice, Solenne and Sylvie.
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* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 15:38, 14 October 2014

Contents

Friday 11th July

Lab work

Fill in the WIKI (Notebook and Protocols)

by Juliette & Romain

The chassis coli Odor free

Preparation of competent cells E.coli

by Maher and Sean

Competent strains used:

  • DH5α
  • MG1655Z1

Sean did the first strain, Maher did the second one.

Protocol

Electrophoresis

Electrophoresis 11.07.2014.jpg

by Fabio and Marie

  1. Cm FNR-RBS-AmilCP term (1)
  2. Cm FNR-RBS-AmilCP term (2)
  3. B0015 (1)
  4. B0015 (2)
  5. PCola-GES 10^-2 (1)
  6. PCola-GES 10^-2 (1)
  7. PCola-GES 10^-2 (2)
  8. PCola-GES 10^-2 (2)
  9. PJBG-6409 10^-2 (1)
  10. PJBG-6409 10^-2 (2)
  11. PJBG-6409 10^-2 (2)

Protocol


Photo of the Day

Paris Saclay 11 july.jpg

Members present:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/11"