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Team:Paris Saclay/Notebook/July/3
From 2014.igem.org
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| + | {{Team:Paris_Saclay/notebook_header}} | ||
=Thursday 3rd July= | =Thursday 3rd July= | ||
| - | |||
==Lab Work== | ==Lab Work== | ||
| - | + | ===- The chassis coli Odor free=== | |
| - | === | + | ====Phages P1 transduction in MG1655 and MG1655Z1==== |
| - | + | ''by Mathias'' | |
| - | + | ||
In 2ml Eppendorf : | In 2ml Eppendorf : | ||
| Line 17: | Line 16: | ||
'''Wait 1 or 2 hours at 37°C with agitation''' | '''Wait 1 or 2 hours at 37°C with agitation''' | ||
| - | Pour in LB Kan petri dishes, incubate at 37°C.(''Turn the petri dishes over when there is no more liquid in!'') | + | Pour in LB Kan petri dishes, incubate at 37°C. (''Turn the petri dishes over when there is no more liquid in!'') |
| + | |||
| + | ===Plasmid DNA Purification=== | ||
| + | ''by Fabio, Maher, Mathieu, Marie and Romain'' | ||
| + | |||
| + | * BBa_K773002 (x2) | ||
| + | * BBa_K517003 (x2) | ||
| + | * BBa_K773002 (x2) | ||
| + | * BBa_K592011 (x2) | ||
| + | * BBa_K1033910 (x2) | ||
| + | * BBa_K592009 (x2) | ||
| + | * BBa_K786002 (x2) | ||
| + | * BBa_K786003 (x2) | ||
| + | * BBa_J45014 (x2) | ||
| + | * BBa_I9026 (x2) | ||
| + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol] | ||
| - | |||
| - | + | ==Photo of the Day== | |
| - | + | [[File:Paris Saclay 3_july.jpg|600px|center]] | |
| + | '''Members present:''' | ||
| + | *Instructors : Alice & Solenne. | ||
| + | *Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain. | ||
| - | + | {{Team:Paris_Saclay/notebook_footer}} | |
Latest revision as of 15:34, 14 October 2014
Contents |
Thursday 3rd July
Lab Work
- The chassis coli Odor free
Phages P1 transduction in MG1655 and MG1655Z1
by Mathias
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation
Pour in LB Kan petri dishes, incubate at 37°C. (Turn the petri dishes over when there is no more liquid in!)
Plasmid DNA Purification
by Fabio, Maher, Mathieu, Marie and Romain
- BBa_K773002 (x2)
- BBa_K517003 (x2)
- BBa_K773002 (x2)
- BBa_K592011 (x2)
- BBa_K1033910 (x2)
- BBa_K592009 (x2)
- BBa_K786002 (x2)
- BBa_K786003 (x2)
- BBa_J45014 (x2)
- BBa_I9026 (x2)
Photo of the Day
Members present:
- Instructors : Alice & Solenne.
- Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
