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Team:Paris Saclay/Notebook/September/10
From 2014.igem.org
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| + | =Wednesday 10th September= | ||
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==Lab Work== | ==Lab Work== | ||
''by Mélanie'' | ''by Mélanie'' | ||
| - | === | + | ===Lemon Scent=== |
====Plasmid extraction==== | ====Plasmid extraction==== | ||
From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/9#PCR Yesterday] I do a plasmid extraction. | From the liquide culture made [https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/September/9#PCR Yesterday] I do a plasmid extraction. | ||
| - | [[File:1009 vérif extraction plasmides.jpg|300px]] | + | [[File:1009 vérif extraction plasmides.jpg|300px|center]] |
these clones (LS GS CAD) had sent to sequencing | these clones (LS GS CAD) had sent to sequencing | ||
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| - | [[File:1009 digestion topo pacI.jpg|200PX]] | + | [[File:1009 digestion topo pacI.jpg|200PX|center]] |
====Purification==== | ====Purification==== | ||
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====culture==== | ====culture==== | ||
As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B | As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B | ||
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| - | |||
==Modeling== | ==Modeling== | ||
| - | |||
''by Arnaud, Eugène, Juliette and Pierre'' | ''by Arnaud, Eugène, Juliette and Pierre'' | ||
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* ''Translecture system'' : maybe we have to give up this part of our work because of the new project. | * ''Translecture system'' : maybe we have to give up this part of our work because of the new project. | ||
* ''Diffusion of the limonene'' : Eugène has focused on the concentration of limonene in the atmosphere. | * ''Diffusion of the limonene'' : Eugène has focused on the concentration of limonene in the atmosphere. | ||
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| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 10_september.jpg|600px|center]] | ||
| + | |||
| + | [http://emmanuelhirsch.fr/ Emmanuel Hirsch] , one of those few we have interviewed during this month of september. | ||
| + | |||
| + | To learn more about the ethic aspect of our project, just click [https://2014.igem.org/Team:Paris_Saclay/Ethics here] | ||
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| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 09:15, 14 October 2014
Contents |
Wednesday 10th September
Lab Work
by Mélanie
Lemon Scent
Plasmid extraction
From the liquide culture made Yesterday I do a plasmid extraction.
these clones (LS GS CAD) had sent to sequencing problem with ps
digestion
I digest with PacI the plasmid that contain LS and GS
| component | volume |
|---|---|
| H2O | 5μl |
| buffer | 3μl |
| PacI | 1μl |
| Plasmid | 20μl |
Purification
Electrophoresis to isolate the insert and purification with a kit = I failed
culture
As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B
Modeling
by Arnaud, Eugène, Juliette and Pierre
Meeting about modeling with advisor Claire to talk about the achievement of our model
- Diffusion of O2 in the gel : maths is OK but biologist need more drawings.
- Bacterial growth on the surface : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab.
- Translecture system : maybe we have to give up this part of our work because of the new project.
- Diffusion of the limonene : Eugène has focused on the concentration of limonene in the atmosphere.
Photo of the Day
[http://emmanuelhirsch.fr/ Emmanuel Hirsch] , one of those few we have interviewed during this month of september.
To learn more about the ethic aspect of our project, just click here
