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Team:Paris Saclay/Notebook/July/11
From 2014.igem.org
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
Revision as of 14:20, 26 August 2014
Contents |
Friday 11th July
Lab work
Fill in the WIKI (Notebook and Protocols)
by Juliette & Romain
A - The chassis coli Odor free
Preparation of competent cells E.coli
by Maher and Sean
Competent strains used:
- DH5α
- MG1655Z1
Sean did the first strain, Maher did the second one.
Electrophoresis
by Fabio and Marie
- Cm FNR-RBS-AmilCP term (1)
- Cm FNR-RBS-AmilCP term (2)
- B0015 (1)
- B0015 (2)
- PCola-GES 10^-2 (1)
- PCola-GES 10^-2 (1)
- PCola-GES 10^-2 (2)
- PCola-GES 10^-2 (2)
- PJBG-6409 10^-2 (1)
- PJBG-6409 10^-2 (2)
- PJBG-6409 10^-2 (2)
Members present:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
