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Team:Paris Saclay/Protocols
From 2014.igem.org
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#[https://2014.igem.org/Team:Paris_Saclay/Protocols/P1_phages_transduction P1 phages transduction] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/P1_phages_transduction P1 phages transduction] | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2 Transformation of competent ''E. coli'' by CaCl<sub>2</sub>] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2 Transformation of competent ''E. coli'' by CaCl<sub>2</sub>] | ||
| + | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/ cloning in pGEMTeasy] | ||
{{Team:Paris_Saclay/protocols_footer}} | {{Team:Paris_Saclay/protocols_footer}} | ||
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Revision as of 17:27, 20 August 2014
Protocols
- Preparation of supercompetent E. coli cells
- Transformation of supercompetent E. coli cells
- Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue
- Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue
- Gel Electrophoresis
- Transformation of CaCl2 supercompetent E. coli cells
- PCR for the genomic DNA of bacteria
- PCR clean-up
- PCR for bacterial culture
- Transformation of competent cells by electroporation
- BioBrick Assembly
- P1 Phages Stock
- P1 phages transduction
- Transformation of competent E. coli by CaCl2
- cloning in pGEMTeasy
