Monday 18th August

Lab work

B - Construction of the fusion protein (color)

PCR of the chromoprotein

27 µl H₂O

10µl 5X phusion buffer

1µl dNTP 10mM

1 µl Primer (iPS83 and iPS84)

small amount of DNA

1,5µl DMSO

0,5µl Phusion

PCR cycle:

98° --> 30 sec

98° --> 10 sec

58° --> 30 sec

72° --> 45 sec

72°1 --> 10 min

D - Lemon Scent

PCR of BBa_K517003

By Hoang Vu and Eugène

We realized a PCR to amplify the β-pinene synthase gene.

PCR of BBa_K762100

by Sean

Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on 8th August).

Primers used: iPS66 and iPS67.

PCR of pCola

by Laetitia and Melanie

We realized a PCR to amplify the geraniol synthase gene.

Five tubes were prepared. Protocol was modified to match the one used for pCola (PCR conducted on 8th August).

Primers used : iPS91bis and iPS82

Electrophoresis of the 3 PCR

(image)

Ligation of PCR products inside pGEMTeasy

by Laetitia

We used the 3 previously PCR products to perform 3 reaction of ligation. The DNA insert used for each was : PS-6, LS-1 and GS-5

16°C until afternoon and 4°C during the night

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