Team:Paris Saclay/Notebook/August/20
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+ | ===Lemon scent=== | ||
+ | |||
+ | ===Bacterial culture=== | ||
+ | ''by Laetitia'' | ||
+ | |||
+ | Here are the results of the bacterial culture of yesterday | ||
+ | [[File:20 08 bacterial culture.jpg| 800px]] | ||
+ | |||
+ | Bacteria with the pGEMTeasy without the DNA insert are blue and the bacteria with the pGEMTeasy and the DNA insert (PS, GS or LS gene) are white | ||
+ | |||
+ | Then, we have chosen 3 white colonies for each DNA insert and we have launched 3 corresponding liquid cultures : | ||
+ | |||
+ | - 5mL LB + ampiciline (1/1000e) | ||
+ | |||
+ | - 1 white colony | ||
+ | |||
+ | The 9 cultures are incubated at 37°C |
Revision as of 11:04, 20 August 2014
Wednesday 20th August
Lemon scent
Bacterial culture
by Laetitia
Here are the results of the bacterial culture of yesterday
Bacteria with the pGEMTeasy without the DNA insert are blue and the bacteria with the pGEMTeasy and the DNA insert (PS, GS or LS gene) are white
Then, we have chosen 3 white colonies for each DNA insert and we have launched 3 corresponding liquid cultures :
- 5mL LB + ampiciline (1/1000e)
- 1 white colony
The 9 cultures are incubated at 37°C