Team:Paris Saclay/Notebook/August/18

From 2014.igem.org

(Difference between revisions)
(→PCR of BBa_K762100)
(→PCR of BBa_K517003)
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We realized a PCR to amplify the β-pinene synthase gene.
We realized a PCR to amplify the β-pinene synthase gene.
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#H<sub>2</sub>O - 29.5 µl
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{| class="wikitable centre" width="50%"
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#Buffer 5X - 10 µl
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|+ 
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#MgCl<sub>2</sub> - 4 µl
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|-
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#dNTP - 1µl
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! scope=col | components
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#IPS68bis (25µM) - 2µl
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! scope=col | volumes
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#IPS69 (25µM) - 2µl
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|-
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#DNA - 1µl
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|DNA GS
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#GoTag enzyme - 0.5µl
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|1 μl
 +
|-
 +
|green goTaq buffer 5X
 +
|10 μl
 +
|-
 +
|GoTag enzyme
 +
|0.5 µl
 +
|-
 +
|H<sub>2</sub>O
 +
|29.5 µl
 +
|-
 +
|MgCl<sub>2</sub>
 +
|4 µl
 +
|-
 +
|dNTP
 +
|1µl
 +
|-
 +
|IPS68bis
 +
|2 µl
 +
|-
 +
|IPS69bis
 +
|2 µl
 +
|}
====PCR of BBa_K762100====
====PCR of BBa_K762100====

Revision as of 14:36, 19 August 2014

Contents

Monday 18th August

Lab work

D - Lemon Scent

PCR of BBa_K517003

By Hoang Vu and Eugène

We realized a PCR to amplify the β-pinene synthase gene.

components volumes
DNA GS 1 μl
green goTaq buffer 5X 10 μl
GoTag enzyme 0.5 µl
H2O 29.5 µl
MgCl2 4 µl
dNTP 1µl
IPS68bis 2 µl
IPS69bis 2 µl

PCR of BBa_K762100

by Sean

Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on 8th August).

Primers used: iPS66 and iPS67.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/August/18"