Team:Paris Saclay/Notebook/August/18

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====PCR of BBa_K762100====
====PCR of BBa_K762100====
''by Sean''
''by Sean''
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Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#PCR 8th August]).  
Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#PCR 8th August]).  

Revision as of 14:18, 19 August 2014

Contents

Monday 18th August

Lab work

D - Lemon Scent

PCR of BBa_K517003

By Hoang Vu and Eugène

We realized a PCR to amplify the β-pinene synthase gene.

  1. H2O - 29.5 µl
  2. Buffer 5X - 10 µl
  3. MgCl2 - 4 µl
  4. dNTP - 1µl
  5. IPS68bis (25µM) - 2µl
  6. IPS69 (25µM) - 2µl
  7. DNA - 1µl
  8. GoTag enzyme - 0.5µl

PCR of BBa_K762100

by Sean

Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on 8th August).

Primers used: iPS66 and iPS67.

Back to the calendar

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