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Team:Paris Saclay/Notebook/August/18
From 2014.igem.org
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==Lab work== | ==Lab work== | ||
===D - Lemon Scent=== | ===D - Lemon Scent=== | ||
| + | |||
| + | ====PCR of BBa-K517003==== | ||
| + | |||
| + | ''By Hoang Vu and Eugène'' | ||
| + | |||
| + | We realized a PCR to amplificate the β-pinene synthase gene. | ||
| + | |||
| + | #H2O - 29.5 µL | ||
| + | #Buffer 5X - 10 µL | ||
| + | #MgCl2 - 4 µL | ||
| + | #dNTP - 1µL | ||
| + | #IPS68bis (25µM) - 2µL | ||
| + | #IPS69 (25µM) - 2µL | ||
| + | #DNA - 1µL | ||
| + | #GoTag enzyme - 0.5µL | ||
====PCR of BBa_K762100==== | ====PCR of BBa_K762100==== | ||
Revision as of 12:54, 18 August 2014
Contents |
Monday 18th August
Lab work
D - Lemon Scent
PCR of BBa-K517003
By Hoang Vu and Eugène
We realized a PCR to amplificate the β-pinene synthase gene.
- H2O - 29.5 µL
- Buffer 5X - 10 µL
- MgCl2 - 4 µL
- dNTP - 1µL
- IPS68bis (25µM) - 2µL
- IPS69 (25µM) - 2µL
- DNA - 1µL
- GoTag enzyme - 0.5µL
PCR of BBa_K762100
Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on 8th August).
Primers used: iPS66 and iPS67.
