Team:Exeter

From 2014.igem.org

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<h1 size="20">Exeter iGEM 2014</h1>
<h1 size="20">Exeter iGEM 2014</h1>
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  <p>Our project has two aims: To create an organism capable of effectively degrading environmental contaminants; and to create and improve synthetic tools for the iGEM database.</p>
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  <p style="font-family:verdana">Our project has two aims: To create an organism capable of effectively degrading environmental contaminants; and to create and improve synthetic tools for the iGEM database.</p>
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                   <p>Our goal was to create a system capable of degrading environmental contaminants. Although we are working with TNT and Nitroglycerin, we aim to create a <b>modular system</b> which can be adapted to work on other chemical pollutants while still <b>utilizing the same regulatory elements.</b></p>
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                   <p style="font-family:verdana">Our first goal is to create a bacterial strain capable of degrading environmental contaminants such as TNT and Nitroglycerin. We are investigating two enzymes that can potentially perform this function, and will study their characteristics both </i>in vivo</i> and <i>in vitro</i>.</p>
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                   <p>We are looking at some of the most commonly used regulators in iGEM to assess how reliable they are, by <b>testing for otherwise unknown combinatorial effects.</b></p>
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                   <p style="font-family:verdana">Our second aim is to study some of the most commonly used regulators (promoters and ribosome binding sequences) found in the iGEM Registry of Standard Parts to assess how reliable they are, by combining them in different contexts. The reliability of such parts is essential when designing and optimizing biological systems.</p>
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                   <p>We are also testing the fluorescent protein iLOV, which we think has the opportunity to become <b>a more effective marker and tool than GFP.</b></p>
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                   <p style="font-family:verdana">In addition to this we are also testing the fluorescent protein iLOV, originally added to the Registry by the Glasgow 2011 Team. iLOV has the potential to become an alternative reporter to the commonly used GFP (and derivatives) in E. coli, but a lack of characterization has hindered its used in iGEM.</p>
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                   <p>These aspects of our project will come together to create an <b>effective, synthetic, modular system capable of effective bioremediation.</b></p>
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                   <p style="font-family:verdana">These aspects of our project will come together to create an <b>effective, synthetic, modular system capable of effective bioremediation.</b></p>
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Revision as of 22:12, 15 August 2014

Exeter | ERASE


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Exeter iGEM 2014

Our project has two aims: To create an organism capable of effectively degrading environmental contaminants; and to create and improve synthetic tools for the iGEM database.

Our first goal is to create a bacterial strain capable of degrading environmental contaminants such as TNT and Nitroglycerin. We are investigating two enzymes that can potentially perform this function, and will study their characteristics both </i>in vivo</i> and in vitro.

Our second aim is to study some of the most commonly used regulators (promoters and ribosome binding sequences) found in the iGEM Registry of Standard Parts to assess how reliable they are, by combining them in different contexts. The reliability of such parts is essential when designing and optimizing biological systems.

In addition to this we are also testing the fluorescent protein iLOV, originally added to the Registry by the Glasgow 2011 Team. iLOV has the potential to become an alternative reporter to the commonly used GFP (and derivatives) in E. coli, but a lack of characterization has hindered its used in iGEM.

These aspects of our project will come together to create an effective, synthetic, modular system capable of effective bioremediation.

Exeter | ERASE