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Team:Paris Saclay/Notebook/July/11
From 2014.igem.org
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=Friday 11th July= | =Friday 11th July= | ||
==Lab work== | ==Lab work== | ||
| - | ===Fill in the WIKI (Notebook and Protocols)=== | + | ====Fill in the WIKI (Notebook and Protocols)==== |
''by Juliette & Romain'' | ''by Juliette & Romain'' | ||
| - | ===Preparation of competent cells E.coli=== | + | ===A - The chassis coli Odor free=== |
| + | ====Preparation of competent cells E.coli==== | ||
''by Maher and Sean'' | ''by Maher and Sean'' | ||
| Line 16: | Line 17: | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
| - | ===Electrophoresis=== | + | ====Electrophoresis==== |
[[File:Electrophoresis_11.07.2014.jpg|400px|right]] | [[File:Electrophoresis_11.07.2014.jpg|400px|right]] | ||
''by Fabio and Marie'' | ''by Fabio and Marie'' | ||
Revision as of 14:30, 7 August 2014
Contents |
Friday 11th July
Lab work
Fill in the WIKI (Notebook and Protocols)
by Juliette & Romain
A - The chassis coli Odor free
Preparation of competent cells E.coli
by Maher and Sean
Competent strains used:
- DH5α
- MG1655Z1
Sean did the first day, Maher did the second one.
Electrophoresis
by Fabio and Marie
- Cm FNR-RBS-AmilCP term (1)
- Cm FNR-RBS-AmilCP term (2)
- B0015 (1)
- B0015 (2)
- PCola-GES 10^-2 (1)
- PCola-GES 10^-2 (1)
- PCola-GES 10^-2 (2)
- PCola-GES 10^-2 (2)
- PJBG-6409 10^-2 (1)
- PJBG-6409 10^-2 (2)
- PJBG-6409 10^-2 (2)
