Team:Paris Saclay/Notebook/July/11
From 2014.igem.org
(Difference between revisions)
(→Friday 11th July) |
|||
Line 1: | Line 1: | ||
+ | {{Team:Paris_Saclay/notebook_header}} | ||
=Friday 11th July= | =Friday 11th July= | ||
- | |||
==Lab work== | ==Lab work== | ||
- | + | ===Fill in the WIKI (Notebook and Protocols)=== | |
- | === | + | |
- | + | ||
''by Juliette & Romain'' | ''by Juliette & Romain'' | ||
- | === | + | ===Preparation of competent cells E.coli=== |
- | + | ||
''by Maher and Sean'' | ''by Maher and Sean'' | ||
Line 19: | Line 16: | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
- | === | + | ===Electrophoresis=== |
- | + | ||
- | + | ||
- | + | ||
[[File:Electrophoresis_11.07.2014.jpg|400px|right]] | [[File:Electrophoresis_11.07.2014.jpg|400px|right]] | ||
+ | ''by Fabio and Marie'' | ||
#Cm FNR-RBS-AmilCP term (1) | #Cm FNR-RBS-AmilCP term (1) | ||
Line 39: | Line 34: | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol] | ||
- | + | {{Team:Paris_Saclay/notebook_footer}} |
Revision as of 08:54, 7 August 2014
Contents |
Friday 11th July
Lab work
Fill in the WIKI (Notebook and Protocols)
by Juliette & Romain
Preparation of competent cells E.coli
by Maher and Sean
Competent strains used:
- DH5α
- MG1655Z1
Sean did the first day, Maher did the second one.
Electrophoresis
by Fabio and Marie
- Cm FNR-RBS-AmilCP term (1)
- Cm FNR-RBS-AmilCP term (2)
- B0015 (1)
- B0015 (2)
- PCola-GES 10^-2 (1)
- PCola-GES 10^-2 (1)
- PCola-GES 10^-2 (2)
- PCola-GES 10^-2 (2)
- PJBG-6409 10^-2 (1)
- PJBG-6409 10^-2 (2)
- PJBG-6409 10^-2 (2)