Team:Paris Saclay/Notebook

From 2014.igem.org

(Difference between revisions)
(→D - Lemon scent)
m (→Labwork)
 
(26 intermediate revisions not shown)
Line 1: Line 1:
-
{{Team:Paris_Saclay/default_header}}
+
{{Team:Paris_Saclay/labwork_header}}
<html>
<html>
     <style>
     <style>
Line 6: Line 6:
</html>
</html>
-
='''Notebook'''=
+
=Labwork=
-
We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.
+
==Notebook==
 +
We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
-
[https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook&action=edit Click here to edit this page]
+
 
<html>
<html>
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/style.css&action=raw&ctype=text/css">
<link rel="stylesheet" type="text/css" href="https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook/style.css&action=raw&ctype=text/css">
Line 16: Line 17:
<div id="notebook_div"></div>
<div id="notebook_div"></div>
</html>
</html>
-
==Planning==
+
{{Team:Paris_Saclay/default_footer}}
-
===A - The chassis coli Odor free===
+
-
*Culture of the supercompetent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
+
-
*Phages preparation for the transduction - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July]
+
-
*Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 3rd July]
+
-
*Cultures of MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 4th July]
+
-
*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 10th July]
+
-
*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 11th July]
+
-
*Preparation of competent cells E. coli MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July]
+
-
*Transformation of supercompetent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July]
+
-
*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July]
+
-
*Preparation and transformation of competent MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 22nd July]
+
-
*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 25th July]
+
-
*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 28th July]
+
-
*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29 29th July]
+
-
*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 30th July]
+
-
*Stria of MG1655 and MG1655Z1 on LB dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 1st August]
+
-
*Stria of MG1655 and MG1655Z1 on LB and kan dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 4th August]
+
-
*PCR verification of the final strains MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 5th August]
+
-
*Final stock of MG1655 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6 6th August]
+
-
 
+
-
===B - Construction of the fusion protein (color)===
+
-
 
+
-
===C - Salicylate Inducible Suppressing System===
+
-
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23rd July]
+
-
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#C_-_Salicylate_Inducible_Suppressing_System 24th July]
+
-
*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#C_-_Salicylate_Inducible_Suppressing_System 25th July]
+
-
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July]
+
-
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July]
+
-
*Main Digestion - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July]
+
-
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July]
+
-
*DNA extraction from gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July]
+
-
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July]
+
-
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July]
+
-
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#C_-_Salicylate_Inducible_Suppressing_System 1st August]
+
-
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
+
-
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
+
-
*Final Stock BBa_K1372000 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
+
-
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#C_-_Salicylate_Inducible_Suppressing_System 5th August]
+
-
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
+
-
*Digestion of BBa_B0015 and BBa_K1372000 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
+
-
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
+
-
*DNA extraction from gel agarose - ''7th August''
+
-
*Ligation - ''7th August''
+
-
*Transformation of competent E.coli cells - ''8th August''
+
-
*Liquid Culture - ''11th August''
+
-
*Plasmid DNA Purification - ''12th August''
+
-
*Digestion to check & Electrophoresis - ''12th August''
+
-
*Final Stock - ''12th August''
+
-
 
+
-
===D - Lemon scent===
+
-
*Rehydration of BioBricks  BBa_J45014, BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
+
-
* Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18#Preparation_of_electrocompetent_DY330_and_transformation_via_pJBEI6409 18th July]
+
-
* Extraction of p cola plasmid DNA - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#Extraction_of_p_cola_plasmid_DNA 22nd July]
+
-
* PCR targeting with DY330 and pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#PCR_Targeting 22nd July]
+
-
* Extraction and electrophoresis of BBa_K762100 with pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Plasmid_DNA_Extraction_&_Electrophoresis 24th July]
+
-
* Gel electrophoresis of p cola - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Gel_electrophoresis_of_p_cola 24th July]
+
-
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Transformation_of_electrocompetent_cells 25th July]
+
-
* Liquid culture of DY330 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Liquid_Culture 28th July]
+
-
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Transformation_of_electrocompetent_cells 29th July]
+
-
* PCR of BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#PCR_of_BBa_K762100 29th July]
+
-
* PCR of BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#PCR_of_BBa_K762100 30th July]
+
-
* Culture of DY330 + pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent 31st July]
+
-
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells 1st August]
+
-
* Electroporation of DY330+pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Electroporation 4th August]
+
-
* PCR of BBa_517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Polymerase_chain_reaction 4th August]
+
-
* Gel electrophoresis of BBa_K517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Electrophoresis 5th August]
+
-
* PCR cleanup of BBa_K517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#PCR_Clean-up 6th August]
+
-
* PCR of BBa_517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Polymerase_chain_reaction 7th August]
+
-
* PCR Clean-up of BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#PCR_Clean-up_of_BBa_K762100 11th August]
+
-
 
+
-
===E - Banana scent===
+
-
 
+
-
===F - The lemon shaping===
+
-
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
+
-
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 5th August]
+
-
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 7th August]
+
-
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 8th August]
+

Latest revision as of 01:48, 18 October 2014

Labwork

Notebook

We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook"