Team:Paris Saclay/Notebook

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='''Notebook'''=
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=Labwork=
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==Notebook==
We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.
We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.
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[https://2014.igem.org/wiki/index.php?title=Team:Paris_Saclay/Notebook&action=edit Click here to edit this page]
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==Planning==
 
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===A - The ''E. coli'' odor free chassis===
 
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*Culture of MG1655 and MG1655Z1 strains- [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
 
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*P1 phage stock preparation for the transduction of the ''Delta-tnaA::Kan'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July]
 
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*P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 3rd July]
 
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*Cultures of MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 4th July]
 
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*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 10th July]
 
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*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 11th July]
 
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*Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July]
 
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*Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with plasmid BT340- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July]
 
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*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July]
 
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*Preparation and transformation of competent MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 22nd July]
 
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*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 25th July]
 
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*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 28th July]
 
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*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29 29th July]
 
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*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with plasmid BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 30th July]
 
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*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 1st August]
 
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*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB and kan dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 4th August]
 
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*PCR verification of the final strains ''Delta-tnaA'' MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 5th August]
 
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*Final stock of MG1655 ''Delta-tnaA'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6 6th August]
 
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===B - Construction of the fusion protein (color)===
 
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===C - Salicylate Inducible Suppressing System===
 
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*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23rd July]
 
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*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#C_-_Salicylate_Inducible_Suppressing_System 24th July]
 
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*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#C_-_Salicylate_Inducible_Suppressing_System 25th July]
 
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*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July]
 
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*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July]
 
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*Main Digestion of BBa_J61051 and BBa_K228001 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July]
 
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*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July]
 
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*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July]
 
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*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July]
 
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*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July]
 
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*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#C_-_Salicylate_Inducible_Suppressing_System 1st August]
 
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*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
 
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*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
 
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*Final Stock '''BBa_K1372000''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#C_-_Salicylate_Inducible_Suppressing_System 4th August]
 
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*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#C_-_Salicylate_Inducible_Suppressing_System 5th August]
 
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*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
 
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*Digestion of BBa_K1372000 and BBa_B0015- [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
 
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*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August]
 
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*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#C_-_Salicylate_Inducible_Suppressing_System 7th August]
 
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*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#C_-_Salicylate_Inducible_Suppressing_System 7th August]
 
-
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#C_-_Salicylate_Inducible_Suppressing_System 8th August]
 
-
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#C_-_Salicylate_Inducible_Suppressing_System 11th August]
 
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*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#C_-_Salicylate_Inducible_Suppressing_System 12th August]
 
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*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#C_-_Salicylate_Inducible_Suppressing_System 12th August]
 
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*Final Stock '''BBa_K1372001''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#C_-_Salicylate_Inducible_Suppressing_System 12th August]
 
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===D - Lemon scent===
 
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*Rehydration of BioBricks  BBa_J45014, BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
 
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* Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18#Preparation_of_electrocompetent_DY330_and_transformation_via_pJBEI6409 18th July]
 
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* Extraction of p cola plasmid DNA - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#Extraction_of_p_cola_plasmid_DNA 22nd July]
 
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* PCR targeting with DY330 and pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#PCR_Targeting 22nd July]
 
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* Extraction and electrophoresis of BBa_K762100 with pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Plasmid_DNA_Extraction_&_Electrophoresis 24th July]
 
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* Gel electrophoresis of p cola - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Gel_electrophoresis_of_p_cola 24th July]
 
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* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Transformation_of_electrocompetent_cells 25th July]
 
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* Liquid culture of DY330 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Liquid_Culture 28th July]
 
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* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Transformation_of_electrocompetent_cells 29th July]
 
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* PCR of BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#PCR_of_BBa_K762100 29th July]
 
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* PCR of BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#PCR_of_BBa_K762100 30th July]
 
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* Culture of DY330 + pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent 31st July]
 
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* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells 1st August]
 
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* Electroporation of DY330+pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Electroporation 4th August]
 
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* PCR of BBa_517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Polymerase_chain_reaction 4th August]
 
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* Gel electrophoresis of BBa_K517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Electrophoresis 5th August]
 
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* PCR cleanup of BBa_K517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#PCR_Clean-up 6th August]
 
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* PCR of BBa_517003 and BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Polymerase_chain_reaction 7th August]
 
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* PCR Clean-up of BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#PCR_Clean-up_of_BBa_K762100 11th August]
 
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* PCR Clean-up of p cola and BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#PCR_Clean-up_of_p_cola_and_BBa_K517003 12th August]
 
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* Gel electrophoresis of p cola, BBa_K762100 and BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Gel_electrophoresis_of_p_cola,_BBa_K762100_and_BBa_K517003 12th August]
 
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* Gel electrophoresis of BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13#Gel_electrophoresis_of_BBa_K762100 13th August]
 
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* Cloning BBa_K517003 and p cola in pGEMTeasy - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13#Cloning_BBa_K517003_and_p_cola_in_pGEMTeasy 13th August]
 
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* PCR of BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18#PCR_of_BBa_K517003 18th August]
 
-
* PCR of BBa_K762100 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18#PCR_of_BBa_K762100 18th August]
 
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* Transformation of DH5α with CAD and chromoprotein - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/21#Transformation_of_DH5.CE.B1_with_CAD_and_chromoprotein 21st August]
 
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* Extraction of pPS3 and pPS4 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26 26th August]
 
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===E - Banana scent===
 
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===F - The lemon shaping===
 
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*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
 
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*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 5th August]
 
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*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 7th August]
 
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*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 8th August]
 
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*Incubation of E. Coli with plasmid FNR RBS AmylCP in LB [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11 11th August]
 
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*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 12th August]
 
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*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13 13th August]
 
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*Preparation of M63 medium [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/14 14th August]
 
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* Incubation of E. Coli with plasmid FNR RBS AmylCP in M63 [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18#Monday_18th_August 18th August]
 
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*Transformation of odor free E. coli with plasmids coding Fluo Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 27th August]
 
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* Results of Fluorescent Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29#Friday_29th_August 29th August]
 
{{Team:Paris_Saclay/default_footer}}
{{Team:Paris_Saclay/default_footer}}

Latest revision as of 01:48, 18 October 2014

Labwork

Notebook

We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook"