Team:Paris Saclay/Notebook/July/9
From 2014.igem.org
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==Lab work== | ==Lab work== | ||
====Plasmid DNA Extraction==== | ====Plasmid DNA Extraction==== | ||
- | + | ''by Romain and Sean'' | |
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B. | Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B. | ||
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====Electrophoresis==== | ====Electrophoresis==== | ||
- | + | ''by Arnaud, Romain and Sean'' | |
- | Plasmids used: | + | Plasmids used: |
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A. | *DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A. | ||
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B. | *DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B. | ||
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====New culture==== | ====New culture==== | ||
- | + | ''by Romain'' | |
New liquid cultures of: | New liquid cultures of: | ||
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* Sponsoring | * Sponsoring | ||
* Wiki's progress | * Wiki's progress | ||
- | * | + | * Modeling's progress |
+ | ==Photo of the Day== | ||
+ | [[File:Paris Saclay 9_july.jpg|600px|center]] | ||
'''Members present:''' | '''Members present:''' | ||
* Instructors and advisors: Alice, Olivier, Solenne and Sylvie. | * Instructors and advisors: Alice, Olivier, Solenne and Sylvie. | ||
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} |
Latest revision as of 13:57, 17 October 2014
Contents |
Wednesday 9th July
Lab work
Plasmid DNA Extraction
by Romain and Sean
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
Electrophoresis
by Arnaud, Romain and Sean
Plasmids used:
- DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
- DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
- XL1-Blue pCola-ges Kan
- pJBEI-6409 Cm
New culture
by Romain
New liquid cultures of:
- FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
- BBa B0015 dble ter pSB1C3 Cm
4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.
Reunion
Conference with the team to discuss about the project. The handled topics were:
- Definition of Biobricks to make
- Planning of internships
- Definition of Biobricks to render
- Understanding the construction of consecutive genes
- Organisation and planning of experiences to do
- Sponsoring
- Wiki's progress
- Modeling's progress
Photo of the Day
Members present:
- Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.