Team:Paris Saclay/Notebook/July/8

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{{Team:Paris_Saclay/notebook_header}}
=Tuesday 8th July=
=Tuesday 8th July=
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==Lab work==
==Lab work==
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====Rehydration of the following biobricks:====
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====1 - Rehydration of the following biobricks:====
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''by Sean''
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'''Sean'''
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* BBa_B0015(CmR)  
* BBa_B0015(CmR)  
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* BBa_J23114(AmpR)
* BBa_J23114(AmpR)
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Protocol:
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Protocol:  
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# Pierce foil cover of well containing biobrick of interest.
# Pierce foil cover of well containing biobrick of interest.
# Add 10 μl of water in well.
# Add 10 μl of water in well.
# Transfer contents of well into a 1.5 ml microcentrifuge tube.
# Transfer contents of well into a 1.5 ml microcentrifuge tube.
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====2 - Transformation in DH5α of the following biobricks:====
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====Transformation in DH5α of the following biobricks:====
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'''Mathieu & Maher'''
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''by Mathieu & Maher''
* BBa_B0015(CmR)
* BBa_B0015(CmR)
* BBa_J23119(CmR)
* BBa_J23119(CmR)
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* Control + : pUC18 (20ng)
* Control + : pUC18 (20ng)
* Control -  
* Control -  
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol]
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NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.
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NB : The protocol was modified. Instead of spreading 10<sup>-1</sup> and 10<sup>-2</sup> solutions of bacteria we took a non diluted solution and a 10<sup>-1</sup> solution.
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====3 - Plasmid DNA preparation:====
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====Plasmid DNA preparation:====
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''by Romain & Sean''
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'''Romain & Sean'''
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Plasmids used:
Plasmids used:
* pJBEI-6409
* pJBEI-6409
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''E. coli'' strain used:
''E. coli'' strain used:
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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==Photo of the Day==
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[[File:Paris Saclay 8_july.jpg|600px|center]]
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'''Members present:'''
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* Instructors and advisors: Alice, Solenne and Sylvie.
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* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
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{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 15:36, 14 October 2014

Contents

Tuesday 8th July

Lab work

Rehydration of the following biobricks:

by Sean

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)

Protocol:

  1. Pierce foil cover of well containing biobrick of interest.
  2. Add 10 μl of water in well.
  3. Transfer contents of well into a 1.5 ml microcentrifuge tube.

Transformation in DH5α of the following biobricks:

by Mathieu & Maher

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)
  • Control + : pUC18 (20ng)
  • Control -

Protocol

NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.

Plasmid DNA preparation:

by Romain & Sean

Plasmids used:

  • pJBEI-6409

E. coli strain used:

  • XL1-Blue

Protocol


Photo of the Day

Paris Saclay 8 july.jpg

Members present:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/8"