Team:Paris Saclay/Notebook/July/8

From 2014.igem.org

(Difference between revisions)
(→Tuesday 8th July)
m (→Tuesday 8th July)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Paris_Saclay/notebook_header}}
=Tuesday 8th July=
=Tuesday 8th July=
-
 
==Lab work==
==Lab work==
 +
====Rehydration of the following biobricks:====
 +
''by Sean''
-
====1 - Rehydration of the following biobricks:====
+
* BBa_B0015(CmR)  
-
'''Sean'''
+
* BBa_J23119(CmR)
-
 
+
* BBa_K731020(CmR)
-
* BBa B0015(CmR)  
+
* BBa_K206000(CmR)
-
* BBa J23119(CmR)
+
* BBa_J04500(CmR)
-
* BBa K731020(CmR)
+
* BBa_J23100(AmpR)
-
* BBa K206000(CmR)
+
* BBa_J23106(AmpR)
-
* BBa J04500(CmR)
+
* BBa_J23114(AmpR)
-
* BBa J23100(AmpR)
+
-
* BBa J23106(AmpR)
+
-
* BBa J23114(AmpR)
+
-
 
+
-
 
+
-
Protocol:
+
 +
Protocol:
# Pierce foil cover of well containing biobrick of interest.
# Pierce foil cover of well containing biobrick of interest.
-
# Add 10 μL of water in well.
+
# Add 10 μl of water in well.
-
# Transfer contents of well into a 1.5 mL microcentrifuge tube.
+
# Transfer contents of well into a 1.5 ml microcentrifuge tube.
-
====2 - Transformation in DH5α of the following biobricks:====
+
====Transformation in DH5α of the following biobricks:====
-
'''Mathieu & Maher'''
+
''by Mathieu & Maher''
-
* BBa B0015(CmR)
+
* BBa_B0015(CmR)
-
* BBa J23119(CmR)
+
* BBa_J23119(CmR)
-
* BBa K731020(CmR)
+
* BBa_K731020(CmR)
-
* BBa K206000(CmR)
+
* BBa_K206000(CmR)
-
* BBa J04500(CmR)
+
* BBa_J04500(CmR)
-
* BBa J23100(AmpR)
+
* BBa_J23100(AmpR)
-
* BBa J23106(AmpR)
+
* BBa_J23106(AmpR)
-
* BBa J23114(AmpR
+
* BBa_J23114(AmpR)
* Control + : pUC18 (20ng)
* Control + : pUC18 (20ng)
* Control -  
* Control -  
 +
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol]
-
[https://2013.igem.org/Team:Paris_Saclay/Protocols/Transformation Protocol]
+
NB : The protocol was modified. Instead of spreading 10<sup>-1</sup> and 10<sup>-2</sup> solutions of bacteria we took a non diluted solution and a 10<sup>-1</sup> solution.
-
NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.
+
====Plasmid DNA preparation:====
-
 
+
''by Romain & Sean''
-
====3 - Plasmid DNA preparation:====
+
-
 
+
-
'''Romain & Sean'''
+
Plasmids used:
Plasmids used:
* pJBEI-6409
* pJBEI-6409
-
 
''E. coli'' strain used:
''E. coli'' strain used:
* XL1-Blue
* XL1-Blue
-
[https://2013.igem.org/Team:Paris_Saclay/extraction Protocol]
+
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol]
 +
 
 +
 
 +
==Photo of the Day==
 +
[[File:Paris Saclay 8_july.jpg|600px|center]]
 +
 
 +
'''Members present:'''
 +
* Instructors and advisors: Alice, Solenne and Sylvie.
 +
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
 +
 
 +
{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 15:36, 14 October 2014

Contents

Tuesday 8th July

Lab work

Rehydration of the following biobricks:

by Sean

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)

Protocol:

  1. Pierce foil cover of well containing biobrick of interest.
  2. Add 10 μl of water in well.
  3. Transfer contents of well into a 1.5 ml microcentrifuge tube.

Transformation in DH5α of the following biobricks:

by Mathieu & Maher

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)
  • Control + : pUC18 (20ng)
  • Control -

Protocol

NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.

Plasmid DNA preparation:

by Romain & Sean

Plasmids used:

  • pJBEI-6409

E. coli strain used:

  • XL1-Blue

Protocol


Photo of the Day

Paris Saclay 8 july.jpg

Members present:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/8"