"
Team:Paris Saclay/Notebook/July/8
From 2014.igem.org
(Difference between revisions)
m (→Tuesday 8th July) |
|||
| (13 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | = | + | {{Team:Paris_Saclay/notebook_header}} |
| + | =Tuesday 8th July= | ||
| + | ==Lab work== | ||
| + | ====Rehydration of the following biobricks:==== | ||
| + | ''by Sean'' | ||
| - | + | * BBa_B0015(CmR) | |
| - | + | * BBa_J23119(CmR) | |
| - | + | * BBa_K731020(CmR) | |
| - | + | * BBa_K206000(CmR) | |
| - | + | * BBa_J04500(CmR) | |
| - | * | + | * BBa_J23100(AmpR) |
| - | * | + | * BBa_J23106(AmpR) |
| - | * | + | * BBa_J23114(AmpR) |
| - | * | + | |
| - | * | + | |
| - | * | + | |
| - | * | + | |
| - | * | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| + | Protocol: | ||
# Pierce foil cover of well containing biobrick of interest. | # Pierce foil cover of well containing biobrick of interest. | ||
| - | # Add 10 | + | # Add 10 μl of water in well. |
| - | # Transfer contents of well into a 1.5 | + | # Transfer contents of well into a 1.5 ml microcentrifuge tube. |
| - | ==== | + | ====Transformation in DH5α of the following biobricks:==== |
| - | '' | + | ''by Mathieu & Maher'' |
| - | * | + | * BBa_B0015(CmR) |
| - | * | + | * BBa_J23119(CmR) |
| - | * | + | * BBa_K731020(CmR) |
| - | * | + | * BBa_K206000(CmR) |
| - | * | + | * BBa_J04500(CmR) |
| - | * | + | * BBa_J23100(AmpR) |
| - | * | + | * BBa_J23106(AmpR) |
| - | * | + | * BBa_J23114(AmpR) |
* Control + : pUC18 (20ng) | * Control + : pUC18 (20ng) | ||
* Control - | * Control - | ||
| + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol] | ||
| - | + | NB : The protocol was modified. Instead of spreading 10<sup>-1</sup> and 10<sup>-2</sup> solutions of bacteria we took a non diluted solution and a 10<sup>-1</sup> solution. | |
| - | + | ====Plasmid DNA preparation:==== | |
| - | + | ''by Romain & Sean'' | |
| - | ==== | + | |
| - | + | ||
| - | '' | + | |
Plasmids used: | Plasmids used: | ||
* pJBEI-6409 | * pJBEI-6409 | ||
| - | |||
''E. coli'' strain used: | ''E. coli'' strain used: | ||
* XL1-Blue | * XL1-Blue | ||
| + | |||
| + | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | ||
| + | |||
| + | |||
| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 8_july.jpg|600px|center]] | ||
| + | |||
| + | '''Members present:''' | ||
| + | * Instructors and advisors: Alice, Solenne and Sylvie. | ||
| + | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
| + | |||
| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 15:36, 14 October 2014
Contents |
Tuesday 8th July
Lab work
Rehydration of the following biobricks:
by Sean
- BBa_B0015(CmR)
- BBa_J23119(CmR)
- BBa_K731020(CmR)
- BBa_K206000(CmR)
- BBa_J04500(CmR)
- BBa_J23100(AmpR)
- BBa_J23106(AmpR)
- BBa_J23114(AmpR)
Protocol:
- Pierce foil cover of well containing biobrick of interest.
- Add 10 μl of water in well.
- Transfer contents of well into a 1.5 ml microcentrifuge tube.
Transformation in DH5α of the following biobricks:
by Mathieu & Maher
- BBa_B0015(CmR)
- BBa_J23119(CmR)
- BBa_K731020(CmR)
- BBa_K206000(CmR)
- BBa_J04500(CmR)
- BBa_J23100(AmpR)
- BBa_J23106(AmpR)
- BBa_J23114(AmpR)
- Control + : pUC18 (20ng)
- Control -
NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.
Plasmid DNA preparation:
by Romain & Sean
Plasmids used:
- pJBEI-6409
E. coli strain used:
- XL1-Blue
Photo of the Day
Members present:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
