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Team:Paris Saclay/Notebook/July/16
From 2014.igem.org
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=Wednesday 16th July= | =Wednesday 16th July= | ||
| - | |||
==Lab work== | ==Lab work== | ||
| - | + | ===Transformation of competent E.coli cells=== | |
| - | === | + | |
| - | + | ||
''by Fabio, Maher and Romain'' | ''by Fabio, Maher and Romain'' | ||
We transformed in DH5α the following Biobrick: | We transformed in DH5α the following Biobrick: | ||
| - | * | + | * BBa_J23119 |
'''Samples:''' | '''Samples:''' | ||
| - | |||
# Concentrated | # Concentrated | ||
# Undiluted | # Undiluted | ||
| - | # Diluted 10 | + | # Diluted 10<sup>-1</sup> |
# Control | # Control | ||
'''Results:''' | '''Results:''' | ||
| - | |||
# Concentrated: 99 colonies | # Concentrated: 99 colonies | ||
# Undiluted: 5 colonies | # Undiluted: 5 colonies | ||
| Line 26: | Line 22: | ||
'''Conclusion:''' | '''Conclusion:''' | ||
| - | |||
Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures. | Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures. | ||
| Line 32: | Line 27: | ||
===2 - Plasmid DNA Purification=== | ===2 - Plasmid DNA Purification=== | ||
| - | |||
''by Mathieu'' | ''by Mathieu'' | ||
| - | * | + | *BBa_J23100 (x2) |
| - | * | + | *BBa_J23106 (x2) |
| - | * | + | *BBa_J23114 (x2) |
from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/15 15th July] | from Bacterial Culture made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/15 15th July] | ||
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==Reunion== | ==Reunion== | ||
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Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning) | Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning) | ||
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''Arnaud represents us'' | ''Arnaud represents us'' | ||
| - | [ | + | ==Photo of the Day== |
| + | [[File:Paris Saclay 16_july.jpg|600px|center]] | ||
| + | |||
| + | '''Members present:''' | ||
| + | * Instructors and advisors: Alice, Solenne and Sylvie. | ||
| + | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
| + | |||
| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 13:45, 14 October 2014
Contents |
Wednesday 16th July
Lab work
Transformation of competent E.coli cells
by Fabio, Maher and Romain
We transformed in DH5α the following Biobrick:
- BBa_J23119
Samples:
- Concentrated
- Undiluted
- Diluted 10-1
- Control
Results:
- Concentrated: 99 colonies
- Undiluted: 5 colonies
- Diluted: 0
- Control: 0
Conclusion: Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.
2 - Plasmid DNA Purification
by Mathieu
- BBa_J23100 (x2)
- BBa_J23106 (x2)
- BBa_J23114 (x2)
from Bacterial Culture made the 15th July
Reunion
Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)
Reunion: Lab meeting to discuss the project. (afternoon)
Meeting with Evry's Team. (night) Arnaud represents us
Photo of the Day
Members present:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
