"
Team:Paris Saclay/Notebook/September/15
From 2014.igem.org
(Difference between revisions)
(→B-B - Construction of the fusion protein (color)) |
m (→Lab Work) |
||
| (4 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{Team:Paris_Saclay/notebook_header}} | ||
| + | =Monday 15th September= | ||
| + | |||
==Lab Work== | ==Lab Work== | ||
''by Mélanie'' | ''by Mélanie'' | ||
| - | === | + | ===lemon scent=== |
====Transformation with the ligation (futur pPS2/4)==== | ====Transformation with the ligation (futur pPS2/4)==== | ||
| Line 14: | Line 17: | ||
PCR of GS : primer with ecoRI and PSTI restriction site | PCR of GS : primer with ecoRI and PSTI restriction site | ||
| - | === | + | ===Construction of the fusion protein (color)=== |
====PCR==== | ====PCR==== | ||
| Line 21: | Line 24: | ||
====transformation for stock of the w6 clone==== | ====transformation for stock of the w6 clone==== | ||
| + | |||
| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 15_september.jpg|600px|center]] | ||
| + | |||
| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 13:13, 14 October 2014
Contents |
Monday 15th September
Lab Work
by Mélanie
lemon scent
Transformation with the ligation (futur pPS2/4)
liquide culture of some PS clone (topo)
Transformation of LS GS CAS (topo) to do stock
PCR pSCBC3 cloning
PCR of GS : primer with ecoRI and PSTI restriction site
Construction of the fusion protein (color)
PCR
We have decided to separate the two protein So I do a PCR to have the yellow and the blue protein and i cloned them in a topo plasmid.
transformation for stock of the w6 clone
Photo of the Day
