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Team:Paris Saclay/Notebook/September/15
From 2014.igem.org
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PCR of GS : primer with ecoRI and PSTI restriction site | PCR of GS : primer with ecoRI and PSTI restriction site | ||
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Revision as of 09:17, 14 October 2014
Contents |
Lab Work
by Mélanie
lemon scent
Transformation with the ligation (futur pPS2/4)
liquide culture of some PS clone (topo)
Transformation of LS GS CAS (topo) to do stock
PCR pSCBC3 cloning
PCR of GS : primer with ecoRI and PSTI restriction site
Construction of the fusion protein (color)
PCR
We have decided to separate the two protein So I do a PCR to have the yellow and the blue protein and i cloned them in a topo plasmid.
transformation for stock of the w6 clone
