Team:Paris Saclay/Notebook/September/15
From 2014.igem.org
(Difference between revisions)
Fanny Boulet (Talk | contribs) |
(→D-lemon scent) |
||
Line 3: | Line 3: | ||
''by Mélanie'' | ''by Mélanie'' | ||
- | === | + | ===lemon scent=== |
====Transformation with the ligation (futur pPS2/4)==== | ====Transformation with the ligation (futur pPS2/4)==== |
Revision as of 09:17, 14 October 2014
Contents |
Lab Work
by Mélanie
lemon scent
Transformation with the ligation (futur pPS2/4)
liquide culture of some PS clone (topo)
Transformation of LS GS CAS (topo) to do stock
PCR pSCBC3 cloning
PCR of GS : primer with ecoRI and PSTI restriction site
B- Construction of the fusion protein (color)
PCR
We have decided to separate the two protein So I do a PCR to have the yellow and the blue protein and i cloned them in a topo plasmid.