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Team:Paris Saclay/Notebook/September/1
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| + | =Monday 1st September= | ||
| + | |||
| + | ==Lab Work== | ||
| + | |||
| + | ===Construction of the fusion protein=== | ||
'by Hoang Vu' | 'by Hoang Vu' | ||
| - | We | + | We picked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent pick out. |
| + | |||
| + | ===Lemon scent=== | ||
| + | |||
| + | '' by melanie'' | ||
| + | |||
| + | ====Limone synthase==== | ||
| + | |||
| + | We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26#Streaking_of_colonies_transformed_by_BBa_K762100.2BpGEMTeasy_.28LS.29 26 August]We do PCR of all the clones: | ||
| + | |||
| + | We use the [https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_bacterial_culture Protocol - PCR for bacterial culture] and we use the same PCR condition than on the [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#PCR August 8] but with the appropriate Primer and Biobrick. | ||
| + | |||
| + | |||
| + | ====PPS5==== | ||
| + | |||
| + | We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) | ||
| + | So we do a PCR in the same condition than [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/20#CAD_PCR August 20] | ||
| + | |||
| + | |||
| + | ====PPS3 and PPS4==== | ||
| + | |||
| + | We need to work on this plasmid tomorrow so we do some culture from the stock | ||
| + | |||
| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 1_september.jpg|600px|center]] | ||
| + | |||
| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 09:13, 14 October 2014
Contents |
Monday 1st September
Lab Work
Construction of the fusion protein
'by Hoang Vu'
We picked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent pick out.
Lemon scent
by melanie
Limone synthase
We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from 26 AugustWe do PCR of all the clones:
We use the Protocol - PCR for bacterial culture and we use the same PCR condition than on the August 8 but with the appropriate Primer and Biobrick.
PPS5
We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) So we do a PCR in the same condition than August 20
PPS3 and PPS4
We need to work on this plasmid tomorrow so we do some culture from the stock
Photo of the Day
