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Team:Paris Saclay/Protocols
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Revision as of 20:37, 4 October 2014
Protocols
Countdown
This page is under Maher's responsibility
- Deadline: 08/oct.
- Uniformisation des textes.
- Rappeler Fabio de creer la photo pour le protocol BioBrick Assembly
- Changer les enzymes du protocol
- Deadline: 12/oct
- Final review.
- Preparation of supercompetent E. coli cells
- Transformation of supercompetent E. coli cells
- Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue
- Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue
- Gel Electrophoresis
- Transformation of CaCl2 supercompetent E. coli cells
- PCR for the genomic DNA of bacteria
- PCR clean-up
- PCR for bacterial culture
- Transformation of competent cells by electroporation
- BioBrick Assembly
- P1 Phages Stock
- P1 phages transduction
- Transformation of competent E. coli by CaCl2
- Cloning in pGEMTeasy
- Electro elution of DNA from agarose gel
